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ExKine™ Nuclear and Cytoplasmic Protein Extraction Kit

ExKine™ Nuclear and Cytoplasmic Protein Extraction Kit

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Specification

Product name ExKine™ Nuclear and Cytoplasmic Protein Extraction Kit
Applications notes Abbkine ExKine™ Nuclear and Cytoplasmic Protein Extraction Kit enable stepwise separation and preparation of crude cytoplasmic and nuclear extracts from mammalian cultured cells or tissue. This Kit is based on allowing cells to swell with hypotonic buffer. And then the cells are disrupted, the cytoplasmic fraction is removed, and the nuclear proteins are released from the nuclei by a high salt buffer. Non-denatured, active proteins are purified in less than two hours.

Product Properties

Kit components • Cytoplasmic Solution A (CES A)
• Cytoplasmic Solution B (CES B)
• Nuclear Extraction Solution (NES)
• DTT (500X)
• Protease Inhibitor (100X)
Features & Benefits • Versatile—suitable for fresh mammalian cultured cells and tissues with little or no cross-contaminations.
• Fast and convenient—non-denatured, active proteins are purified in less than two hours.
• Compatible—apply in various downstream assays, including Western blotting, gel-shift assays, protein assays, reporter gene assays and enzyme activity assays.
Usage notes Perform all steps at 2–8 °C. Use precooled buffers and equipment. Ensure all the solutions are defrosted and homogeneous.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The preparation of an extract from nuclei is often the first step in studying nuclear proteins and their interactions. The resulting preparation can be used directly in the Electrophoresis Mobility Shift Assay (EMSA), footprinting analysis, transcription assays, or as a starting point for the purification of regulatory proteins.

Image & description

Fig.1. Western blot of specific proteins fraction using Abbkine ExKine™ Nuclear and Cytoplasmic Protein Extraction Kit. Sample: 293T cells lysate. C: cytoplasmic extraction was analyzed using α-tubulin antibody (A01080). N: nuclear extraction was analyzed using α-tubulin antibody (A01080).

Fig.1. Western blot of specific proteins fraction using Abbkine ExKine™ Nuclear and Cytoplasmic Protein Extraction Kit. Sample: 293T cells lysate. C: cytoplasmic extraction was analyzed using α-tubulin antibody (A01080). N: nuclear extraction was analyzed using α-tubulin antibody (A01080).

Fig.2. Western blot of specific proteins fraction using Abbkine ExKine™ Nuclear and Cytoplasmic Protein Extraction Kit. Sample: 293T cells lysate. C: cytoplasmic extraction was analyzed using Histone H3 antibody (A01070). N: nuclear extraction was analyzed using Histone H3 antibody (A01070).

Fig.2. Western blot of specific proteins fraction using Abbkine ExKine™ Nuclear and Cytoplasmic Protein Extraction Kit. Sample: 293T cells lysate. C: cytoplasmic extraction was analyzed using Histone H3 antibody (A01070). N: nuclear extraction was analyzed using Histone H3 antibody (A01070).

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