|Product name||ERK1/2 Mouse Monoclonal Antibody (1H4)|
|Immunogen||Synthetic Peptide of ERK1/2 at AA range of 140-220|
|Reactivity||Human, Mouse, Rat|
|Applications notes||Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:1000-1:2000), IHC-P (1:100-1:200).|
|Preparation method||The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen|
|Storage buffer||PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.|
|Storage instructions||Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||MAPK1 (mitogen-activated protein kinase 1) encodes a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. One study also suggests that this protein acts as a transcriptional repressor independent of its kinase activity. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for MAPK1.|
|Others||ERK1/2 protein detects endogenous levels of MAPK1/MAPK3.|
Fig.1. Immunohistochemical analysis of paraffin-embedded Human Lung Carcinoma Tissue using ERK1/2 Mouse mAb diluted at 1:200.
Fig.2. Immunohistochemical analysis of paraffin-embedded Human Colon Carcinoma Tissue using ERK1/2 Mouse mAb diluted at 1:200.
Fig.3. Western blot analysis of 1) Hela Cell Lysate, 2) Mouse Brain Tissue Lysate, 3) Rat Brain Tissue Lysate using ERK1/2 Mouse mAb diluted at 1:2000.
Author：Dawei Wu , Dongwei Lv, et al Publication name：Endocrine-Related Cancer IF：4.50