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ERK 1 Polyclonal Antibody

ERK 1 Polyclonal Antibody

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Specification

Product name ERK 1 Polyclonal Antibody
Immunogen Synthesized peptide derived from the Internal region of human ERK 1 at AA range: 130-210
Host Rabbit
Reactivity Human, Mouse, Rat
Applications ELISA, IF, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IF (1:200-1:1000), ELISA (1:40000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative MAPK3; ERK1; PRKM3; Mitogen-activated protein kinase 3; MAP kinase 3; MAPK 3; ERT2; Extracellular signal-regulated kinase 1; ERK-1; Insulin-stimulated MAP2 kinase; MAP kinase isoform p44; p44-MAPK; Microtubule-associated protein 2 kinase; p

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Mitogen-activated protein kinase 3 encoded by MAPK3 is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described.
Gene ID 5595
Alternative MAPK3; ERK1; PRKM3; Mitogen-activated protein kinase 3; MAP kinase 3; MAPK 3; ERT2; Extracellular signal-regulated kinase 1; ERK-1; Insulin-stimulated MAP2 kinase; MAP kinase isoform p44; p44-MAPK; Microtubule-associated protein 2 kinase; p
Others ERK 1 Polyclonal Antibody detects endogenous levels of ERK 1 protein.
Accession P27361
Observed Band(KD) 45

Image & description

Fig.1. Western Blot analysis of various cells using ERK 1 Polyclonal Antibody diluted at 1:500.

Fig.1. Western Blot analysis of various cells using ERK 1 Polyclonal Antibody diluted at 1:500.

Fig.2. Western Blot analysis of 3T3 cells using ERK 1 Polyclonal Antibody diluted at 1:500.

Fig.2. Western Blot analysis of 3T3 cells using ERK 1 Polyclonal Antibody diluted at 1:500.

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