| Product name | ER81 Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from the N-terminal region of human ER81 at AA range: 10-90 |
| Host | Rabbit |
| Reactivity | Human,Mouse |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:10000;IF 1:50-200 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | ETV1; ER81; ETS translocation variant 1; Ets-related protein 81 |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 60kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | ETV1 encodes a member of the ETS (E twenty-six) family of transcription factors. The ETS proteins regulate many target genes that modulate biological processes like cell growth, angiogenesis, migration, proliferation and differentiation. All ETS proteins contain an ETS DNA-binding domain that binds to DNA sequences containing the consensus 5'-CGGA[AT]-3'. The protein encoded by this gene contains a conserved short acidic transactivation domain (TAD) in the N-terminal region, in addition to the ETS DNA-binding domain in the C-terminal region. This gene is involved in chromosomal translocations, which result in multiple fusion proteins including EWS-ETV1 in Ewing sarcoma and at least 10 ETV1 partners (see PMID: 19657377, Table 1) in prostate cancer. In addition to chromosomal rearrangement, this gene is overexpressed in prostate cancer, melanoma and gastrointestinal stromal tumor. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. |
| Gene ID | 2115 |
| Alternative | ETV1; ER81; ETS translocation variant 1; Ets-related protein 81 |
| Others | ER81 Polyclonal Antibody detects endogenous levels of ER81 protein. |
| Accession | P50549 |
| Observed Band(KD) | 60 |
Fig.1. Western Blot analysis of various cells using ER81 Polyclonal Antibody.
Fig.2. Western Blot analysis of HuvEc cells using ER81 Polyclonal Antibody.
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