| Product name | ELNE Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from part region of human ELNE protein |
| Host | Rabbit |
| Reactivity | Human,Mouse,Bovine, |
| Applications | WB,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000;ELISA 1:5000-20000 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | Neutrophil elastase; Bone marrow serine protease; Elastase-2; Human leukocyte elastase; HLE; Medullasin; PMN elastase |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 29kD |
| Storage buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode structurally similar proteins. Elastase, Neutrophil Expressed is proteolytically processed to generate the active protease. Following activation, this protease hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix. The enzyme may play a role in degenerative and inflammatory diseases through proteolysis of collagen-IV and elastin. Elastase, Neutrophil Expressed also degrades the outer membrane protein A (OmpA) of E.coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in ELANE are associated with cyclic neutropenia and severe congenital neutropenia (SCN). ELANE is present in a gene cluster on chromosome 19. |
| Gene ID | 1991 |
| Alternative | Neutrophil elastase; Bone marrow serine protease; Elastase-2; Human leukocyte elastase; HLE; Medullasin; PMN elastase |
| Others | The antibody detects endogenous levels of ELNE protein |
| Accession | P08246 |
| Observed Band(KD) | 29 |
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