| Product name | EliKine™ Triiodothyronine (T3) ELISA Kit |
| Reactivity | Bovine, Chicken, Dog, Goat, Human, Monkey, Mouse, Rabbit, Rat, Sheep, Swine |
| Applications | ELISA |
| Applications notes | EliKine™ T3 ELISA Kit employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to T3. Standards or samples are added to the appropriate microtiter plate wells with Biotin-conjugated T3. A competitive inhibition reaction is launched between T3 (Standards or samples) and Biotin-conjugated T3 with the pre-coated antibody specific for T3. The more amount of T3 in samples, the less antibody bound by Biotin-conjugated T3. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of T3 in the sample. The color development is stopped and the intensity of the color is measured. |
| Detection method | Colorimetric |
| SampleType | Other biological fluids, Plasma, Serum |
| Assay type | Competitive ELISA (quantitative) |
| Assay duration | Multiple steps standard competitive ELISA assay with a working time of 3-4 hours. It depends on the experience of the operation person. |
| Kit components | • T3 microplate • T3 standard • Biotin conjugated T3 • Avidin-HRP • HRP substrate A • HRP substrate B • Stop solution • Wash buffer • Plate covers. |
| Features & Benefits | EliKine™ T3 ELISA Kit has high sensitivity and excellent specificity for detection of T3. No significant cross-reactivity or interference between T3 and analogues was observed. |
| Calibration range | 0.5 ng/ml-8 ng/ml |
| Limit of detection | 0.4 ng/ml |
| Usage notes | • Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for |
| Storage instructions | The unopened kit should be stored at 2 - 8 °C. After opening, please store refer to protocols. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Triiodothyronine (T3) is a useful marker for the diagnosis of hypothyroidism and hyperthyroidism. The level of T3 is decreased in hypothyroid patients and is increased in hyperthyroid patients. Production of T3 is activated by thyroid-stimulating hormone (TSH), which is released from the anterior pituitary gland. This pathway is part of a closed-loop feedback process: Elevated concentrations of T3 in the blood plasma inhibit the production of TSH in the anterior pituitary gland. As concentrations of these hormones decrease, the anterior pituitary gland increases production of TSH, and by these processes, a feedback control system stabilizes the amount of thyroid hormones that are in the bloodstream. |
Fig.1. T3 Standard Curve.
Fig.2. EliKine™ T3 ELISA Kit is a competitive ELISA to quantitate T3 in samples.
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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