| Product name | EliKine™ Rat TNF-α ELISA Kit |
| Reactivity | Rat |
| Applications | ELISA |
| Applications notes | EliKine™ Rat TNF-α ELISA Kit employs a two-site sandwich ELISA to quantitate TNF-α in samples. An antibody specific for TNF-α has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNF-α present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNF-α is added to the wells. After washing, proprietary EliKine™ Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNF-α bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Detection method | Colorimetric |
| SampleType | Cell culture supernatants, Other biological fluids, Plasma, Serum |
| Assay type | Sandwich ELISA (quantitative) |
| Assay duration | Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person. |
| Alternative | TNF-α; TNF; DADB-70P7.1; DIF; TNF-alpha; TNFA; TNFSF2; APC1 protein; TNF superfamily; member 2; TNF; macrophage-derived; TNF; monocyte-derived; cachectin; tumor necrosis factor alpha; TNFSF7 |
| Kit components | • Rat TNF-α microplate • Rat TNF-α standard • Rat TNF-α detect antibody • EliKine™ Streptavidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers. |
| Features & Benefits | EliKine™ Rat TNF-α ELISA Kit has high sensitivity and excellent specificity for detection of Rat TNF-α. No significant cross-reactivity or interference between Rat TNF-α and analogues was observed. |
| Calibration range | 31.25 pg/mL- 2000 pg/mL |
| Limit of detection | 16 pg/ml |
| Usage notes | • Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate. |
| Storage instructions | The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Tumor necrosis factor acts as a cytokine, binding TNF receptors and plays a role in regulation of cell proliferation, induction of apoptosis, and inflammatory response. |
| Gene ID | 24835 |
| Alternative | TNF-α; TNF; DADB-70P7.1; DIF; TNF-alpha; TNFA; TNFSF2; APC1 protein; TNF superfamily; member 2; TNF; macrophage-derived; TNF; monocyte-derived; cachectin; tumor necrosis factor alpha; TNFSF7 |
| Accession | P16599 |
Fig.1. Rat TNF-α Standard Curve.
Fig.2. EliKine™ Rat TNF-α ELISA Kit is a sandwich ELISA to quantitate TNFA in samples.
Author：R Suri, TC Nag, N Mehra, YR Neupane, S Shafi Publication name：Journal of Drug Delivery IF：6.819
Author：Wang X, Chang L, Chen L, et al Publication name：Journal of Ethnopharmacology IF：5.4
Author：Batra P, Shende P Publication name：Colloids and Surfaces A: Physicochemical and Engineering Aspects IF：3.99
Author：N Ziroglu, A Koluman, B Kaleci, B Tanriverdi Publication name：Injury IF：2.687
Author：Daniel Priscilla, et al. Publication name：Bioinformation IF：1.9
Author：D Singh Publication name：eurchembull IF：1.704
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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