| Product name | EliKine™ Human Leptin ELISA Kit |
| Reactivity | Human |
| Applications | ELISA |
| Applications notes | EliKine™ Human Leptin ELISA Kit employs a two-site sandwich ELISA to quantitate Leptin in samples. An antibody specific for Leptin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Leptin present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Leptin is added to the wells. After washing, proprietary EliKine™ Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Leptin bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Detection method | Colorimetric |
| SampleType | Cell culture supernatants, Other biological fluids, Plasma, Serum |
| Assay type | Sandwich ELISA (quantitative) |
| Assay duration | Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person. |
| Alternative | FLJ94114; LEP; LEP_HUMAN; LEPD; Leptin; Leptin Precursor Obesity Factor; OB; Obese protein |
| Kit components | • Human Leptin microplate • Human Leptin standard • Human Leptin detect antibody • EliKine™ Streptavidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers. |
| Features & Benefits | EliKine™ Human Leptin ELISA Kit has high sensitivity and excellent specificity for detection of Human Leptin . No significant cross-reactivity or interference between Human Leptin and analogues was observed. |
| Calibration range | 31.25 pg/mL- 2000 pg/mL |
| Limit of detection | 16 pg/mL |
| Usage notes | • Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate. |
| Storage instructions | The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | LEP gene encodes a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in LEP gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. LEP gene has also been linked to type 2 diabetes mellitus development. |
| Gene ID | 3952 |
| Alternative | FLJ94114; LEP; LEP_HUMAN; LEPD; Leptin; Leptin Precursor Obesity Factor; OB; Obese protein |
| Accession | P41159 |
Fig.1. Human Leptin Standard Curve.
Fig.2. EliKine™ Human Leptin ELISA Kit is a sandwich ELISA to quantitate LEP in samples.
Author：Mugundhan, Varsha, et al. Publication name：HIV Nursing IF：2.3
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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