| Product name | EliKine™ Human Carcinoembryonic Antigen (CEA) ELISA Kit |
| Reactivity | Human |
| Applications | ELISA |
| Applications notes | EliKine™ Human Carcino-embryonic antigen (CEA) ELISA Kit employs a double antibody sandwich method to quantitate Human CEA in samples. An antibody specific for Human CEA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Human CEA present is bound by the immobilized antibody. After removing any unbound substances, a HRP-conjugated antibody specific for Human CEA is added to the wells. After washing, remove any unbound HRP-conjugated antibody reagent, adding HRP Substrate (TMB), TMB turns blue under the catalysis of HRP, and turns yellow after adding stop solution. Measure the OD value with a microplate reader at 450 nm wavelength. The Human carcino-embryonic antigen (CEA) concentration is proportional to the OD450 nm value. |
| Detection method | Colorimetric |
| SampleType | Cell culture supernatants, Plasma, Serum |
| Assay type | Sandwich ELISA (quantitative) |
| Assay duration | Multiple steps standard sandwich ELISA assay with a working time of 3.5 hours. It depends on the experience of the operation person. |
| Alternative | Carcinoembryonic antigen ELISA Kit; Carcinoembryonic antigen-related cell adhesion molecule 5 ELISA Kit; CD66e ELISA Kit; CEA ELISA Kit; Ceacam5 ELISA Kit; CEAM5_HUMAN ELISA Kit. |
| Kit components | • Human CEA Microplate • Human CEA Standard (lyophilized) • Sample Diluent (5×) • Assay Buffer (5×) • HRP-conjugated Human CEA Detect Antibody (100×) • HRP Substrate（TMB） • Stop Solution • Wash Buffer (20x ) • Plate Covers |
| Features & Benefits | EliKine™ Human Carcino-embryonic antigen (CEA) ELISA Kit has high sensitivity and excellent specificity for detection of Human Carcino-embryonic antigen (CEA). No significant cross-reactivity or interference between Human Carcino-embryonic antigen (CEA) and analogues was observed. |
| Calibration range | 0.625 ng/mL-40 ng/mL |
| Limit of detection | 0.313 ng/mL |
| Usage notes | • 1. If Sample Diluent (5×) and Assay Buffer (5×) appears to turn yellow or a small amount of precipitation, etc., it is caused by the serum contained in the reagent. Please centrifuge to remove the precipitate, which will not affect normal use. • 2. Do not mix or substitute reagents with those from other lots or sources. • 3. To avoid cross-contamination, change pipette tips between additions of each standard level, between sample additions, and between reagent additions. Also, use separate reservoirs for each reagent. • 4. To ensure accurate results, proper adhesion of plate covers during incubation steps is necessary. • 5. Stop Solution has certain Corrosive. Please take protective measures when operating. |
| Storage instructions | Stored at 4℃ for 12 months |
| Shipping | Gel pack with blue ice. |
| Precautions | The reagent is only used in the field of scientific research, not suitable for clinical diagnosis or other purposes. |
| Background | Carcino-embryonic antigen (CEA) is a glycoprotein produced by colorectal cancer tissue. It is widely distributed in endoembryo-derived cancer of the digestive system, in the digestive canal tissues of normal embryos, and in a small amount in normal human serum. Carcinoembryonic antigen (CEA) is a broad-spectrum tumor marker, which can reflect the existence of a variety of tumors. It is a good tumor marker for the judgment of efficacy, disease development, monitoring and prognosis of colorectal cancer, breast cancer and lung cancer, but its specificity and sensitivity are not high, and its role in early diagnosis of tumors is not obvious. |
| Alternative | Carcinoembryonic antigen ELISA Kit; Carcinoembryonic antigen-related cell adhesion molecule 5 ELISA Kit; CD66e ELISA Kit; CEA ELISA Kit; Ceacam5 ELISA Kit; CEAM5_HUMAN ELISA Kit. |
Figure. Standard Curve of Human Carcino-embryonic antigen (CEA) in 96-well plate assay, data provided for demonstration purposes only.
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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