| Product name | EliKine™ Human bFGF ELISA Kit |
| Reactivity | Human |
| Applications | ELISA |
| Applications notes | EliKine™ Human bFGF ELISA Kit employs a two-site sandwich ELISA to quantitate bFGF in samples. An antibody specific for bFGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any bFGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for bFGF is added to the wells. After washing, proprietary EliKine™ Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of bFGF bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Detection method | Colorimetric |
| SampleType | Cell culture supernatants, Other biological fluids, Plasma, Serum |
| Assay type | Sandwich ELISA (quantitative) |
| Assay duration | Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person. |
| Alternative | Basic fibroblast growth factor; BFGF; FGF 2; FGF B; FGF2; FGF2 basic; FGFB; Fibroblast growth factor 2 (basic); HBGF 2; HBGF2; HBGH 2; Heparin binding growth; factor 2; Heparin binding growth factor 2 precursor; OTTMUSP00000046776; Prostatropin |
| Kit components | • Human bFGF microplate • Human bFGF standard • Human bFGF detect antibody • Avidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers. |
| Features & Benefits | EliKine™ Human bFGF ELISA Kit has high sensitivity and excellent specificity for detection of Human bFGF. No significant cross-reactivity or interference between Human bFGF and analogues was observed. |
| Calibration range | 15.6 pg/mL-1000 pg/mL |
| Limit of detection | 8 pg/mL |
| Usage notes | • Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate. |
| Storage instructions | The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | The protein encoded by FGF2 gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. |
| Gene ID | 2247 |
| Alternative | Basic fibroblast growth factor; BFGF; FGF 2; FGF B; FGF2; FGF2 basic; FGFB; Fibroblast growth factor 2 (basic); HBGF 2; HBGF2; HBGH 2; Heparin binding growth; factor 2; Heparin binding growth factor 2 precursor; OTTMUSP00000046776; Prostatropin |
| Accession | P09038 |
Fig.1. Human bFGF Standard Curve.
Fig.2. EliKine™ Human bFGF ELISA Kit is a sandwich ELISA to quantitate BFGF in samples.
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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