| Product name | EF-2 Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from human EF-2 around the non-phosphorylation site of T56 |
| Host | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC 1:100-1:300;IF 1:200-1:1000;ELISA 1:5000;Not yet tested in other applications. |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | EEF2; EF2; Elongation factor 2; EF-2 |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 100kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | EEF2 encodes a member of the GTP-binding translation elongation factor family. Eukaryotic translation elongation factor 2 is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation. |
| Gene ID | 1938 |
| Alternative | EEF2; EF2; Elongation factor 2; EF-2 |
| Others | EF-2 Polyclonal Antibody detects endogenous levels of EF-2 protein. |
| Accession | P13639 |
| Observed Band(KD) | 100 |
Fig.1. Western Blot analysis of various cells using EF-2 Polyclonal Antibody diluted at 1:2000.
Fig.2. Western Blot analysis of VEC cells using EF-2 Polyclonal Antibody diluted at 1:2000.
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