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eEF2K (phospho Ser366) Polyclonal Antibody

eEF2K (phospho Ser366) Polyclonal Antibody

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Specification

Product name eEF2K (phospho Ser366) Polyclonal Antibody
Immunogen Synthesized peptide derived from human eEF2K around the phosphorylation site of S366
Host Rabbit
Reactivity Human, Rat
Applications ELISA, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:40000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative EEF2K; Eukaryotic elongation factor 2 kinase; eEF-2 kinase; eEF-2K; Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background EEF2K encodes a highly conserved protein kinase (eukaryotic elongation factor 2 kinase) in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of eukaryotic elongation factor 2 kinase is increased in many cancers and may be a valid target for anti-cancer treatment.
Gene ID 29904
Alternative EEF2K; Eukaryotic elongation factor 2 kinase; eEF-2 kinase; eEF-2K; Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase
Others Phospho-eEF2K (S366) Polyclonal Antibody detects endogenous levels of eEF2K protein only when phosphorylated at S366.
Accession O00418
Observed Band(KD) 105

Image & description

Fig. Western Blot analysis of various cells using Phospho-eEF2K (S366) Polyclonal Antibody diluted at 1:1000.

Fig. Western Blot analysis of various cells using Phospho-eEF2K (S366) Polyclonal Antibody diluted at 1:1000.

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