| Product name | Dynamin I Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from human Dynamin I around the non-phosphorylation site of S774 |
| Host | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:40000;IF 1:50-200 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | DNM1; DNM; Dynamin-1 |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 97kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | DNM1 encodes a member of the dynamin subfamily of GTP-binding proteins. Dynamin 1 possesses unique mechanochemical properties used to tubulate and sever membranes, and is involved in clathrin-mediated endocytosis and other vesicular trafficking processes. Actin and other cytoskeletal proteins act as binding partners for the encoded protein, which can also self-assemble leading to stimulation of GTPase activity. More than sixty highly conserved copies of the 3' region of this gene are found elsewhere in the genome, particularly on chromosomes Y and 15. Alternatively spliced transcript variants encoding different isoforms have been described. |
| Gene ID | 1759 |
| Alternative | DNM1; DNM; Dynamin-1 |
| Others | Dynamin I Polyclonal Antibody detects endogenous levels of Dynamin I protein. |
| Accession | Q05193 |
| Observed Band(KD) | 97 |
Fig.1. Western Blot analysis of various cells using Dynamin I Polyclonal Antibody.
Fig.2. Western Blot analysis of 3T3 cells using Dynamin I Polyclonal Antibody.
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