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DyLight 488, Goat Anti-Mouse IgG

Product Information

  • Product name

    DyLight 488, Goat Anti-Mouse IgG

  • Immunogen

    Mouse IgG whole molecule

  • Host


  • Reactivity


  • Applications

    FCM, ICC, IF

  • Application notes

    Optimal working dilutions should be determined experimentally by the investigator. Suggested starting 1:50-1:1000 dilutions for most fluorescent applications.

  • Clonality


  • Isotype

    Goat IgG

  • Purification

    Affinity purified using solid phase Mouse IgG (H&L) with finally > 95% purity based on SDS-PAGE

  • Conjugate

    Dylight 488

Fig.1. DyLight fluorescent dyes are a new family of dyes with improved brightness. DyLight 488-antibody conjugates are brighter than Cy2 and FITC conjugates and similar in brightness to Alexa Fluor 488 conjugates. DyLight 549-antibody conjugates shows brighter influoresence than TRITC conjugates. Also, DyLight 594-antibody conjugates are noticeably brighter than Alexa 594 conjugates, and much brighter and more water soluble than Texas Red conjugates.

Fig.2. DyLight fluorescent dyes show improved photostability as well. DyLight 488 conjugates fade less than FITC and Cy2 conjugates in mounting media indicating that the DyLight 488 molecule is inherently more photostable in epifluorescence microscopes. DyLight 549 conjugates are about as photostable as Alexa 555 conjugates and slightly more photostable than Cy3 conjugates.

Fig.3. To use the DyLight Fluors with fluorescent imagers, use a spectral line of the blue laser diode for DyLight 405, a cyan (488 nm) laser for DyLight 488, a green (526 nm) laser for DyLight 550 and 594, and a red (633 nm) laser for DyLight 633 and 650. The DyLight 680, 755 and 800 fluors are compatible with laser- and filter-based infrared imaging instruments that emit in the 700 nm, 750 nm and 800 nm region of the spectrum, respectively.

Product Properties

  • Formulation

    Liquid solution

  • Features & Benefits

    DyLight fluorescent dyes are a new family of dyes with improved brightness and photostability). They are better than or comparable to the best fluorescent dyes from other companies). The detection level of any fluorophore-antibody conjugate depends on brightness and photostability of the dye; antibody activity, specificity, and cross-reactivity; and the optimal moles of dye per antibody). A molar saturation curve vs fluorescence intensity, antibody activity, background level, and/or other parameters has been established for each dye to optimize the level of antibody detection and minimize background). DyLight fluorescent dyes are highly water soluble and remain fluorescent from pH 4 to pH 9).

  • Storage buffer

    Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative, 1% BSA as stablizer and 50% Glycerol.

  • Storage instructions

    Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

  • Shipping

    Gel pack with blue ice.

  • Precautions

    The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

  • Background

    Abbkine secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. We offer high quality secondary antibodies from goat, rabbit and donkey sources for your each application. Serum adsorbed secondary antibodies are also available and are recommended for use with immunoglobulin-rich samples.

  • Others

    The antibody reacts with whole molecule mouse IgG. It also reacts with heavy chains of mouse IgG, and light chains of all other mouse immunoglobulins. It has no reactivity on non-immunoglobulin serum proteins, while it may cross-react with immunoglobulins from other species.

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Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.

Preparation methods for Biochemical

Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.

  1. ♦ What and how to make a "good" buffer?

Antibody application protocols

Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.

  1. ♦ Antibody Western Blotting (WB) protocol
  2. ♦ Antibody Immunohistochemistry (IHC) protocol
  3. ♦ Antibody Immunofluorescence (IF) protocol
  4. ♦ Antibody Immunoprecipitation (IP) protocol
  5. ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol

Protein&peptide usage suggestions

Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.

  1. ♦ Handling and storage suggestion for peptides and protein
  2. ♦ Cytokines and growth factors for cell culture application

Commonly used assay kits guidelines

Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.

We hope this will be helpful for your research work. Please let us know through if you need more information or support.

  1. Immunopotentiator thymosin alpha-1 attenuates inflammatory pain by modulating the Wnt3a/β-catenin pathway in spinal cord

    Jiahua Huang, Huaqing Jiang. NeuroReport 2020, 31:69–75

  2. SIRT6 inhibits cholesterol crystal-induced vascular endothelial dysfunction via Nrf2 activation

    Z Jin, Y Xiao, F Yao. Experimental Cell Research

  3. FEN1 inhibitor increases sensitivity of radiotherapy in cervical cancer cells

    Jin Li Li. Cancer Medicine

  4. Dengue Virus M Protein Promotes NLRP3 Inflammasome Activation To Induce Vascular Leakage in Mice

    P Pan, Q Zhang, W Liu. JOURNAL OF VIROLOGY

  5. Activation of liver X receptor promotes hippocampal neurogenesis and improves long‐term cognitive function recovery in acute cerebral ischemia‐reperfusion mice

    L Chen, D Song, B Chen. Journal of Neurochemistry

  6. The Binding of PD-L1 and Akt Facilitates Glioma Cell Invasion Upon Starvation via Akt/Autophagy/F-Actin Signaling

    RQ Chen, XH Xu, F Liu. Frontiers in Oncology

  7. Dengue Virus Infection Activates Interleukin-1β to Induce Tissue Injury and Vascular Leakage

    P Pan, Q Zhang, W Liu. Frontiers in Microbiology

  8. Surface display of hirame novirhabdovirus (HIRRV) G protein in Lactococcus lactis and its immune protection in flounder (Paralichthys olivaceus)

    L Zhao, X Tang, X Sheng. Microbial cell factories

  9. Polyethylene microplastics affect the distribution of gut microbiota and inflammation development in mice

    B Li, Y Ding, X Cheng. Chemosphere

  10. Manipulation of Mitophagy by “All-in-One” nanosensitizer augments sonodynamic glioma therapy

    F Qu, P Wang, K Zhang. Autophagy

  11. Dimethylated histone H3 lysine 9 is dispensable for the interaction between developmental pluripotency-associated protein 3 (Dppa3) and ten-eleven translocation 3 (Tet3) in somatic cells

    QQ Wang, YM Zhang, X Zhong, JW Li, XR An. Reproduction, Fertility and Development 31(2) 347-356, 2019.

  12. Long non-coding RNA PVT1 promotes autophagy as ceRNA to target ATG3 by sponging microRNA-365 in hepatocellular carcinoma

    L Yang, X Peng, H Jin, J Liu - Gene, 2019 - Elsevier

  13. MicroRNA-155 Suppresses Mesangial Cell Proliferation and TGF-β1 Production via Inhibiting CXCR5-ERK Signaling Pathway in Lupus Nephritis

    J Kong, L Li, Z Lu, J Song, J Yan, J Yang, Z Gu, Z Da. Inflammation, 2019.

  14. Hyperforin improves post-stroke social isolation‑induced exaggeration of PSD and PSA via TGF-β

    Y Zhang, P Yu, H Liu, H Yao. International Journal of Molecular Medicine, 2019.

  15. Protective effect of DMDD, isolated from the root of Averrhoa carambola L., on high glucose induced EMT in HK-2 cells by inhibiting the TLR4-BAMBI-Smad2/3 signaling pathway

    H Zhang, X Wei, S Lu, X Lin, J Huang, L Chen. Biomedicine & Pharmacotherapy, 2019.

  16. Unravelling the receptor binding property of egg drop syndrome virus (EDSV) from the crystal structure of EDSV fiber head

    Y Song, Q Wei, Y Liu, H Feng, Y Chen, Y Wang.International Journal of Biological Macromolecules.Volume 139, 15 October 2019, Pages 587-595.

  17. Spectrin-based membrane skeleton supports ciliogenesis

    R Jia, D Li, M Li, Y Chai, Y Liu, Z Xie, W Shao. PLoS biology, 2019.

  18. Licorice isoliquiritigenin-encapsulated mesoporous silica nanoparticles for osteoclast inhibition and bone loss prevention

    X Sun, J Zhang, Z Wang, B Liu, S Zhu, L Zhu.Theranostics. 2019; 9(18): 5183–5199.

  19. Hyperforin Promotes Post-stroke Neuroangiogenesis via Astrocytic IL-6-Mediated Negative Immune Regulation in the Ischemic Brain

    Hua Yao, Yujing Zhang, Huaqing Shu, et al. Front Cell Neurosci. 2019 May 7;13:201.

  20. ALDH2 Activation Inhibited Cardiac Fibroblast-to-Myofibroblast Transformation Via the TGF-β1/Smad Signaling Pathway

    Yuan, Qiuhuan, Cao, Shengchuan, Dong, Qianqian, et al. J Cardiovasc Pharmacol. 2019 Apr;73(4):248-256.

  21. Dexmedetomidine alleviates LPS-induced apoptosis and inflammation in macrophages by eliminating damaged mitochondria via PINK1 mediated mitophagy

    Yanyan Wang, Xing Mao, Hongguang Chen, et al. Int Immunopharmacol. 2019 Jun 3;73:471-481.

  22. Cholesterol induces epithelial-to-mesenchymal transition of prostate cancer cells by suppressing degradation of EGFR through APMAP

    Siyuan Jiang, Xuetong Wang, Dalong Song, et al. Cancer Res. 2019 Jun 15;79(12):3063-3075.

  23. HIF‐1α facilitates osteocyte‐mediated osteoclastogenesis by activating JAK2/STAT3 pathway in vitro

    Jie Zhu, Yi Tang, Qing Wu, et al. J Cell Physiol. 2019 Apr 29. doi: 10.1002/jcp.28721.

  24. Fluoxetine delays the cognitive function decline and synaptic changes in a transgenic mouse model of early Alzheimer's disease

    Chun‐ni Zhou, Feng‐lei Chao, Yi Zhang, et al. J Comp Neurol. 2019 Jun 1;527(8):1378-1387.

  25. Allicin attenuates calcium oxalate crystal deposition in the rat kidney by regulating gap junction function

    Yongchang Lai, Xiongfa Liang, Fangling Zhong, et al. J Cell Physiol. 2019 Jun;234(6):9640-9651.

  26. Hyperforin improves post-stroke social isolation‑induced exaggeration of PSD and PSA via TGF-β.

    YUJING ZHANG, PEIYUN YU, HONG LIU, et al. Int J Mol Med. 2019 Jan;43(1):413-425.

  27. Melatonin Enhances Proliferation and Modulates Differentiation of Neural Stem Cells Via Autophagy in Hyperglycemia

    HAOYUAN LI, YANMIN ZHANG, SHANGMING LIU, et al. Stem Cells. 2019 Apr;37(4):504-515.

  28. Quantitative study of the capillaries within the white matter of the Tg2576 mouse model of Alzheimer's disease

    Yi Zhang, Feng‐lei Chao, Lei Zhang, et al. Brain Behav. 2019 Apr;9(4):e01268.

  29. Acute exposure to triphenyl phosphate (TPhP) disturbs ocular development and muscular organization in zebrafish larvae

    Qipeng Shi, Mirabelle M.P.Tsui, ChenyanHu, et al. Ecotoxicol Environ Saf. 2019 Apr 25;179:119-126.

  30. Molecular evolution and characterization of novel Seneca Valley virus (SVV) strains in South China

    Minmin Wang, Lulu Chen, Shuonan Pan, et al. Infect Genet Evol. 2019 Apr;69:1-7

  31. Mandibular osteotomy‐induced hypoxia enhances osteoclast activation and acid secretion by increasing glycolysis

    Yi Tang, Jie Zhu, Danqing Huang, et al. J Cell Physiol. 2019 Jul;234(7):11165-11175.

  32. Effect of DJ-1 on the neuroprotection of astrocytes subjected to cerebral ischemia/reperfusion injury

    Li Peng, Yipeng Zhao, et, al. Journal of Molecular Medicine.February 2019, Volume 97, Issue 2, pp 189–199.

  33. Treatment of donor cells with recombinant KDM4D protein improves preimplantation development of cloned ovine embryos

    Yumei Zhang, Qianqian Wang, et al. Cytotechnology. (2018).

  34. Non-invasive Vagus Nerve Stimulation Protects Against Cerebral Ischemia/Reperfusion Injury and Promotes Microglial M2 Polarization Via Interleukin-17A Inhibition

    Xiao-Ping Zhao, Yuan Zhao, et al. Journal of Molecular Neuroscience. (2018).

  35. Dimethylated histone H3 lysine 9 is dispensable for the interaction between developmental pluripotency-associated protein 3 (Dppa3) and ten-eleven translocation 3 (Tet3) in somatic cells

    Qian-Qian Wang , Yu-Mei Zhang, et al. Reproduction, Fertility and Development. (2018).

  36. Establishment and characterization of a carcinoma-associated fibroblast cell line derived from a human salivary gland adenoid cystic carcinoma

    Jing Kong, Han Zhao, et al. Cell Communication & Adhesion. 2018; 24(1): 11-18.

  37. Stereological Investigation of the Effects of Treadmill Running Exercise on the HippocampalNeurons in Middle-Aged APP/PS1 Transgenic Mice.

    Chao Fenglei, Jiang Lin, et al. Journal of Alzheimer's Disease. 2018; 63(2): 689-703.

  38. Invasion of midgut epithelial cells by a persistently transmitted virus is mediated by sugartransporter 6 in its insect vector.

    Faliang Qin, Wenwen Liu, et al. PLoS Pathogens. 2018; 14(7): e1007201.

  39. Cullin1 represses systematic inflammasome activation by binding and catalyzing NLRP3 ubiquitination

    Pin Wan, Qi Zhang, et al. bioRxiv. (2018)

  40. Potential role of CXCL10/CXCR3 signaling in the development of morphine tolerance in periaqueductal gray

    WeiWanga, YawenPeng, et al. Neuropeptides. 2018; 65: 120-127.

  41. MicroRNA-155 Suppresses Mesangial Cell Proliferation and TGF-β1 Production via Inhibiting CXCR5-ERK Signaling Pathway in Lupus Nephritis

    Kong, J., Li, L., Lu, Z. et al. Inflammation. (2018). 

  42. Mitochondrial Ferritin Is a Hypoxia-Inducible Factor 1α-Inducible Gene That Protects from Hypoxia-Induced Cell Death in Brain

    Wu Q, Wu W S, Su L, et al. Antioxid Redox Signal. 2018.

  43. Neuroglobin boosts axon regeneration during ischemic reperfusion via p38 binding and activation depending on oxygen signal

    Xiong XX, Pan F, Chen RQ, et al. Cell Death Dis, 2018, 9(2): 163.

  44. The effect of cavernous nerve traction on erectile function in rats

    Li H, Chen L, Wang T, et al. PLoS One, 2017, 12(10): e0186078.

  45. Calpain inhibition improves erectile function in diabetic mice via upregulating endothelial nitric oxide synthase expression and reducing apoptosis

    Li H, Chen LP, Wang T, et al. Asian J Androl, 2018.

  46. Thioredoxin-interacting protein links endoplasmic reticulum stress to inflammatory brain injury and apoptosis after subarachnoid haemorrhage

    Zhao Q, Che X, Zhang H, et al. J Neuroinflammation, 2017, 14(1): 105.

  47. Periodontal ligament-like tissue regeneration with drilled porous decalcified dentin matrix sheet composite

    Feng G, Wu Y, Yu Y, et al. Oral Dis, 2018, 24(3): 429-441.

  48. XRCC2-Deficient Cells are Highly Sensitive to 5-Fluorouracil in Colorectal Cancer

    Zhang Y Z, An J H, Liu Y, et al. Cell Physiol Biochem, 2017, 43(3): 1207-1219.

  49. Retigabine ameliorates acute stress-induced impairment of spatial memory retrieval through regulating USP2 signaling pathways in hippocampal CA1 area

    Li C, Zhang J, Xu H, et al. Neuropharmacology, 2018, 1; 135: 151-163.

  50. Porcine enterocyte protein Btnl5 negatively regulates NF-kappa B pathway by interfering p65 nuclear translocationGene2018,10;646:47-55

    Bao Q, Li C, Xu C, et al.

  51. Radiation-induced G2/M arrest rarely occurred in glioblastoma stem-like cells

    Liu J, Liu Y, Xie T, et al. Int J Radiat Biol, 2018, 94(4): 394-402.

  52. Deproteinized bovine bone matrix induces osteoblast differentiation via macrophage polarization

    Shi M, Wang C, Wang Y, et al. J Biomed Mater Res A, 2018, 106(5): 1236-1246.

  53. Liver X receptors agonist promotes differentiation of rat bone marrow derived mesenchymal stem cells into dopaminergic neuron-like cells

    Cheng O, Tian X, Luo Y, et al. 2017, Oncotarget.

  54. Integrated Proteomic and Transcriptomic Analysis Reveals Long Noncoding RNA HOX Transcript Antisense Intergenic RNA (HOTAIR) Promotes Hepatocellular Carcinoma Cell Proliferation by Regulating Opioid Growth Factor Receptor (OGFr)

    Wu Y, Xiong Q, Li S T, et al. MOLECULAR & CELLULAR PROTEOMICS, 2018, 17: 146-159.

  55. Knockdown of Bone Morphogenetic Proteins Type 1a Receptor (BMPR1a) in Breast Cancer Cells Protects Bone from Breast Cancer-Induced Osteolysis by Suppressing RANKL Expression

    Liu Y A, Zhang R X, Yuan W, et al. Cellular Physiology and Biochemistry, 2018, 45, 1759-1771.

  56. The dual role of poly(ADP-ribose) polymerase-1 in modulating parthanatos and autophagy under oxidative stress in rat cochlear marginal cells of the stria vascularis

    Jiang H Y, Yang Y, Zhang Y Y, et al. Redox Biology, 2018, 14: 361-370.

  57. Cullin1 represses systematic inflammasome activation by binding and catalyzing NLRP4 ubiquitination

    Wan P, Zhang Q, Liu W Y, et al. BioRxiv, 2018.

  58. Resolvase OsGEN1 mediates DNA repair by homologous recombination

    Wang, Chong, et al. Plant physiology (2017): pp-01726.

  59. Secretogranin-II Plays A Critical Role In Zebrafish Neurovascular Modeling

    Tao, Binbin, et al. bioRxiv (2017): 123125.

  60. Coaxial 3D bioprinting of self-assembled multicellular heterogeneous tumor fibers

    Dai, Xingliang, et al. Scientific Reports 7 (2017).

  61. Recruitment of CD11b+Ly6C+ monocytes in non-small cell lung cancer xenografts challenged by anti-VEGF antibody

    Chen, Xie‑Wan, et al. Oncology Letters 14.1 (2017): 615-622.

  62. Klotho suppresses tumor progression via inhibiting IGF-1R signaling in T‑cell lymphoma

    Zhou, Xiangxiang, et al. Oncology Reports (2017).

  63. Ten-Eleven Translocation-2 (Tet2) Is Involved in Myogenic Differentiation of Skeletal Myoblast Cells in Vitro

    Zhong, Xia, et al. Scientific Reports 7 (2017): 43539.

  64. Activation of EphA1-Epha receptor axis attenuates diabetic nephropathy in mice

    Li, Yihui, et al. Biochemical and Biophysical Research Communications 486.3 (2017): 693-699.

  65. Claudin-1 regulates pulmonary artery smooth muscle cell proliferation through the activation of ERK1/2

    Cheng, Xiandong, et al. Biomedicine & Pharmacotherapy 89 (2017): 983-990.

  66. A nano-scaled and multi-layered recombinant fibronectin/cadherin chimera composite selectively concentrates osteogenesis-related cells and factors to aid bone repair

    Xing, Junchao, et al. Acta Biomaterialia (2017).

  67. Retinoid acid-induced microRNA-27b-3p impairs C2C12 myoblast proliferation and differentiation by suppressing α-dystrobrevin

    Li, Nan, et al. Experimental Cell Research 350.2 (2017): 301-311.

  68. Role of Wnt/β-catenin signaling pathway in the repair of intestinal mucosa associated with crypt stem cell in a rat model of abdominal compartment syndrome

    Lu T, Luo H, Liu J, et al. Int J Clin Exp Pathol, 2017, 10(2): 2351-2362

  69. Yap1 promotes the survival and self-renewal of breast tumor initiating cells via inhibiting Smad3 signaling

    Sun, Jian-Guo, et al. Oncotarget 7.9 (2016): 9692.

  70. Rifampicin Attenuated Global Cerebral Ischemia Injury via Activating the Nuclear Factor Erythroid 2-Related Factor Pathway

    Chen, B, et al. Frontiers in Cellular Neuroscience 10(2016)

  71. Role of thioredoxin reductase 1 in dysplastic transformation of human breast epithelial cells triggered by chronic oxidative stress

    Dong, Chaoran, et al. Scientific Reports 6(2016): 36860.

  72. Involvement of caspase-3/PTEN signaling pathway in isoflurane-induced decrease of self-renewal capacity of hippocampal neural precursor cells

    Chen, Xin, et al. Brain Research 1625 (2015): 275-286.

  73. Bexarotene protects against traumatic brain injury in mice partially through apolipoprotein E.

    Zhong J, Cheng C, Liu H, et al. Neuroscience, 2016.

  74. The important role of the receptor for activated C kinase 1 (RACK1) in nasopharyngeal carcinoma progression.

    Peng H, Gong P G, Li J B, et al. Journal of translational medicine, 2016, 14(1): 1.

  75. The role of connexin43 in hemorrhagic transformation after thrombolysis in vivo and in vitro.

    Yang X, Chu H, Tang Y, et al. Neuroscience, 2016, 329: 54-65.

  76. Baclofen ameliorates spatial working memory impairments induced by chronic cerebral hypoperfusion via up-regulation of HCN2 expression in the PFC in rats.

    Luo P, Chen C, Lu Y, et al. Behavioural brain research, 2016, 308: 6-13.

  77. Inhibition of interleukin-6 abolishes the promoting effects of pair housing on post-stroke neurogenesis.

    Meng C, Zhang J C, Shi R L, et al. Neuroscience, 2015, 307: 160-170.

  78. XRCC2 as a predictive biomarker for radioresistance in locally advanced rectal cancer patients undergoing preoperative radiotherapy.

    Qin C J, Song X M, Chen Z H, et al. Oncotarget, 2015, 6(31): 32193.

  79. Secretion of adipocytes and macrophages under conditions of inflammation and/or insulin resistance and effect of adipocytes on preadipocytes under these conditions.

    Wei Y T, Xia D S, Yang W K, et al. Biochemistry (Moscow), 2014, 79(7): 663-671.

Cat #: A23210

  • Size
  • Price
  • 100μl $40
  • 500μl $160

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