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Dsg1 Polyclonal Antibody

Dsg1 Polyclonal Antibody

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Specification

Product name Dsg1 Polyclonal Antibody
Immunogen Synthesized peptide derived from part region of human Dsg1 protein at amino acid sequence of 30-90
Host Rabbit
Reactivity Human, Mouse, Rat
Applications ELISA, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:500-1:200), ELISA (1:10000-1:20000).
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen
Alternative Desmoglein-1; Cadherin family member 4; Desmosomal glycoprotein 1; DG1; DGI; Pemphigus foliaceus antigen

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol as stabilizer.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background DSG1 encodes a member of the desmoglein protein subfamily. Desmogleins, along with desmocollins, are cadherin-like transmembrane glycoproteins that are major components of the desmosome. Desmosomes are cell-cell junctions that help resist shearing forces and are found in high concentrations in cells subject to mechanical stress. DSG1 is found in a cluster with other desmoglein family members on chromosome 18. The encoded protein has been identified as a target of auto-antibodies in the autoimmune skin blistering disease pemphigus foliaceus. Disruption of DSG1 has also been associated with the skin diseases palmoplantar keratoderma and erythroderma.
Gene ID 1828
Alternative Desmoglein-1; Cadherin family member 4; Desmosomal glycoprotein 1; DG1; DGI; Pemphigus foliaceus antigen
Others The antibody detects endogenous levels of Dsg1
Accession Q02413
Observed Band(KD) 160

Image & description

Fig.1. Western blot analysis of 293T lysate, antibody was diluted at 1:1000. HRP, Goat Anti-Rabbit IgG (Cat #: A21020) secondary antibody was diluted at 1:20000.

Fig.1. Western blot analysis of 293T lysate, antibody was diluted at 1:1000. HRP, Goat Anti-Rabbit IgG (Cat #: A21020) secondary antibody was diluted at 1:20000.

Fig.2. Immunohistochemical analysis of paraffin-embedded human-skin, antibody was diluted at 1:200.

Fig.2. Immunohistochemical analysis of paraffin-embedded human-skin, antibody was diluted at 1:200.

Fig.3. Immunohistochemical analysis of paraffin-embedded human-skin, antibody was diluted at 1:200.

Fig.3. Immunohistochemical analysis of paraffin-embedded human-skin, antibody was diluted at 1:200.

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