| Product name | Dkk-1 Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from the C-terminal region of human Dkk-1 |
| Host | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC: 1:100-1:300;ELISA 1:10000;IF 1:50-200 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | DKK1; Dickkopf-related protein 1; Dickkopf-1; Dkk-1; hDkk-1; SK |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 29kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | DKK1 encodes dickkopf WNT signaling pathway inhibitor 1 that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma. |
| Gene ID | 22943 |
| Alternative | DKK1; Dickkopf-related protein 1; Dickkopf-1; Dkk-1; hDkk-1; SK |
| Others | Dkk-1 Polyclonal Antibody detects endogenous levels of Dkk-1 protein. |
| Accession | O94907 |
| Observed Band(KD) | 29 |
Fig.1. Western Blot analysis of hela cells using Dkk-1 Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.
Fig.2. Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:100.
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