| Product name | Cytokeratin 8 (Acetyl Lys483) Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from the human Cytokeratin 8 around the acetylation site of K483 |
| Host | Rabbit |
| Reactivity | Human,Rat,Mouse, |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC: 1:100-300;ELISA 1:20000;IF 1:50-200 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | KRT8; CYK8; Keratin, type II cytoskeletal 8; Cytokeratin-8; CK-8; Keratin-8; K8; Type-II keratin Kb8 |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 53kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | KRT8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of KRT8 typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. Keratin 8 plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in KRT8 cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for KRT8. |
| Gene ID | 3856 |
| Alternative | KRT8; CYK8; Keratin, type II cytoskeletal 8; Cytokeratin-8; CK-8; Keratin-8; K8; Type-II keratin Kb8 |
| Others | Acetyl-Cytokeratin 8 (K483) Polyclonal Antibody detects endogenous levels of Cytokeratin 8 protein only when acetylated at K483. |
| Accession | P05787 |
| Observed Band(KD) | 53 |
Fig.1. Western Blot analysis of A549 cells using Acetyl-Cytokeratin 8 (K483) Polyclonal Antibody. Secondary Antibody was diluted at 1:20000.
Fig.2. Western Blot analysis of A549 cells using Acetyl-Cytokeratin 8 (K483) Polyclonal Antibody. Secondary Antibody was diluted at 1:20000.
Fig.3. Immunohistochemical analysis of paraffin-embedded human-breast, antibody was diluted at 1:100.
You must be logged in to post a review.
Reviews
There are no reviews yet.