| Product name | Cytokeratin 14/17 Polyclonal Antibody |
| Immunogen | Synthetic peptide from human protein at AA range: 261-310 |
| Host | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000;ELISA 1:10000-20000 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | KRT14 |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 52kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | KRT14 (keratin 14) encodes a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11. |
| Gene ID | 3861 |
| Alternative | KRT14 |
| Others | The antibody detects endogenous Cytokeratin 14/17 protein. |
| Accession | P02533/Q04695 |
| Observed Band(KD) | 52 |
Fig.1. Western Blot analysis of HEPG2 cells using Antibody diluted at 1:1000. Secondary antibody (catalog#: A21020) was diluted at 1:20000.
Fig.2. Immunohistochemical analysis of paraffin-embedded human-skin, antibody was diluted at 1:200.
Fig.3. Immunohistochemical analysis of paraffin-embedded human-skin, antibody was diluted at 1:200.
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