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Cyclin E1 Polyclonal Antibody

Cyclin E1 Polyclonal Antibody

Views(44) Publications(0) Catalog no(ABP0078)
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Specification

Product name Cyclin E1 Polyclonal Antibody
Immunogen Synthesized peptide derived from the Internal region of human Cyclin E1 at AA range: 60-140
Host Rabbit
Reactivity Human, Mouse, Rat
Applications ELISA, IF, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IF (1:200-1:1000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative CCNE1; CCNE; G1/S-specific cyclin-E1

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Molecular weight 49 KD
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Cyclin E1 encoded by CCNE1 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. Cyclin E1 accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of CCNE1 has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. Cyclin E1 was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB.
Gene ID 898
Alternative CCNE1; CCNE; G1/S-specific cyclin-E1
Others Cyclin E1 Polyclonal Antibody detects endogenous levels of Cyclin E1 protein.
Accession P24864

Image & description

Fig.1. Western Blot analysis of various cells using Cyclin E1 Polyclonal Antibody diluted at 1:500.

Fig.1. Western Blot analysis of various cells using Cyclin E1 Polyclonal Antibody diluted at 1:500.

Fig.2. Immunofluorescence analysis of rat lung tissue. 1, Cyclin E1 Polyclonal Antibody (red) was diluted at 1:200 (4°,overnight). 2, Cy3 labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

Fig.2. Immunofluorescence analysis of rat lung tissue. 1, Cyclin E1 Polyclonal Antibody (red) was diluted at 1:200 (4°,overnight). 2, Cy3 labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

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