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Cyclin B1 Polyclonal Antibody

Cyclin B1 Polyclonal Antibody

Views(1001) Publications(0) Catalog no(ABP51081)
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Specification

Product name Cyclin B1 Polyclonal Antibody
Immunogen Synthesized peptide derived from human Cyclin B1 around the non-phosphorylation site of S126
Host Rabbit
Reactivity Human, Mouse, Rat
Applications ELISA, IF, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:20000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative CCNB1; CCNB; G2/mitotic-specific cyclin-B1

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Cyclin B1 encoded by CCNB1 is a regulatory protein involved in mitosis. The gene product complexes with p34 (cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.
Gene ID 891
Alternative CCNB1; CCNB; G2/mitotic-specific cyclin-B1
Others Cyclin B1 Polyclonal Antibody detects endogenous levels of Cyclin B1 protein.
Accession P14635
Observed Band(KD) 60

Image & description

Fig.1. Western Blot analysis of various cells using Cyclin B1 Polyclonal Antibody diluted at 1:500.

Fig.1. Western Blot analysis of various cells using Cyclin B1 Polyclonal Antibody diluted at 1:500.

Fig.2. Immunohistochemical analysis of paraffin-embedded human uterus tissue. 1, Cyclin B1 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.2. Immunohistochemical analysis of paraffin-embedded human uterus tissue. 1, Cyclin B1 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.3. Immunohistochemical analysis of paraffin-embedded mouse heart tissue. 1, Cyclin B1 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.3. Immunohistochemical analysis of paraffin-embedded mouse heart tissue. 1, Cyclin B1 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.4. Immunohistochemical analysis of paraffin-embedded rat heart tissue. 1, Cyclin B1 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.4. Immunohistochemical analysis of paraffin-embedded rat heart tissue. 1, Cyclin B1 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.5. Immunofluorescence analysis of mouse kidney tissue. 1, Cyclin B1 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

Fig.5. Immunofluorescence analysis of mouse kidney tissue. 1, Cyclin B1 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

Fig.6. Immunofluorescence analysis of rat liver tissue. 1, Cyclin B1 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

Fig.6. Immunofluorescence analysis of rat liver tissue. 1, Cyclin B1 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

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