- Product name
Cy3, Goat Anti-Mouse IgG
Mouse IgG whole molecule
FCM, ICC, IF
- Application notes
Optimal working dilutions should be determined experimentally by the investigator. Suggested 1:50-1:1000 for most fluorescent applications.
Affinity purified using solid phase Mouse IgG (H&L) with finally > 95% purity based on SDS-PAGE
- Storage buffer
Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative, 1% BSA as stablizer and 50% Glycerol.
- Storage instructions
Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Gel pack with blue ice.
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Abbkine secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. We offer high quality secondary antibodies from goat, rabbit and donkey sources for your each application. Serum adsorbed secondary antibodies are also available and are recommended for use with immunoglobulin-rich samples.
The antibody reacts with whole molecule mouse IgG. It also reacts with heavy chains of mouse IgG, and light chains of all other mouse immunoglobulins. It has no reactivity on non-immunoglobulin serum proteins, while it may cross-react with immunoglobulins from other species.
Most popular with customers
Application: ELISA, IHC, WB
Application: FCM, ICC, IF
Application: FCM, ICC, IF
Application: FCM, ICC, IF
Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.
Preparation methods for Biochemical
Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.
Antibody application protocols
Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.
- ♦ Antibody Western Blotting (WB) protocol
- ♦ Antibody Immunohistochemistry (IHC) protocol
- ♦ Antibody Immunofluorescence (IF) protocol
- ♦ Antibody Immunoprecipitation (IP) protocol
- ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol
Protein&peptide usage suggestions
Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.
- ♦ Handling and storage suggestion for peptides and protein
- ♦ Cytokines and growth factors for cell culture application
Commonly used assay kits guidelines
Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.
We hope this will be helpful for your research work. Please let us know through email@example.com if you need more information or support.
Efficient treatment of sporothrix globosa infection using the antibody elicited by recombinant phage nanofibers
F Chen, R Jiang, S Dong, B Yan. Frontiers in pharmacology, 2019.
Decreasing cell population of individual Candida species does not impair the virulence of Candida albicans and Candida glabrata mixed biofilms
Q Li, J Liu, J Shao, W Da, G Shi, T Wang. Frontiers in Microbiology, 2019.
DL-3-n-butylphthalide protects the blood-brain barrier against ischemia/hypoxia injury via upregulation of tight junction proteins
Ye, Zhan-Ying, Xing, Han-Ying, Wang, Bei, et al. Chin Med J (Engl). 2019 Jun 5;132(11):1344-1353.
Telbivudine antagonizes TLR4 to inhibit the epithelial-to-mesenchymal transition in human proximal tubular epithelial cells in vitro
Jie Chen, Detian Li, Enjie Luo. Int Immunopharmacol. 2019 Jun 17;74:105683.
3D bioprinted glioma cell‐laden scaffolds enriching glioma stem cells via epithelial–mesenchymal transition
Xuanzhi Wang, Xingliang Dai, Xinzhi Zhang, et al. J Biomed Mater Res A. 2019 Feb;107(2):383-391.
Efficient Treatment of Sporothrix globosa Infection Using the Antibody Elicited by Recombinant Phage Nanofibers
Feng Chen, Rihua Jiang, Shuai Dong and Bailing Yan. Front Pharmacol. 2019 Feb 27;10:160.
Melatonin protects diabetic heart against ischemia-reperfusion injury, role of membrane receptor-dependent cGMP-PKG activation
Yu L M, Di W C, Dong X, et al. Biochim Biophys Acta, 2018, 1864(2): 563-578.
Coaxial 3D bioprinting of self-assembled multicellular heterogeneous tumor fibers
Dai, Xingliang, et al. Scientific Reports 7 (2017).
Early-Life Exposure to Lead Induces Cognitive Impairment in Elder Mice Targeting SIRT1 Phosphorylation and Oxidative Alterations
Zhang, Lijie, et al. Frontiers in Physiology 8 (2017): 446.
A multifunctional probe for ICP-MS determination and multimodal imaging of cancer cells
Yang, Bin, et al. Biosensors and Bioelectronics 96 (2017): 77-83.
Recombinant Phage Elicits Protective Immune Response against Systemic S. globosa Infection in Mouse Model
Chen, Feng, et al. globosa Infection in Mouse Model
Elemental-tagged immunoassay combined with inductively coupled plasma mass spectrometry for the detection of tumor cells using a lead sulfide nanoparticle label
Yang, Bin, et al. Talanta 167 (2017): 499-505.
The mechanically activated p38/MMP-2 signaling pathway promotes bone marrow mesenchymal stem cell migration in rats
Yang, Zihui, et al. Archives of Oral Biology 76 (2017): 55-60.
The Crystal Structure of the Fifth Scavenger Receptor Cysteine-Rich Domain of Porcine CD163 Reveals an Important Residue Involved in Porcine Reproductive and Respiratory Syndrome Virus Infection
Ma, H, et al. Journal of Virology (2016):JVI.01897-16.
Involvement of caspase-3/PTEN signaling pathway in isoflurane-induced decrease of self-renewal capacity of hippocampal neural precursor cells
Chen, Xin, et al. Brain Research 1625 (2015): 275-286.
Rice ragged stunt virus-induced apoptosis affects virus transmission from its insect vector, the brown planthopper to the rice plant
Huang, Hai-Jian, et al. Scientific reports 5 (2015).
Curcumin induces M2 macrophage polarization by secretion IL-4 and/or IL-13.
Gao S, Zhou J, Liu N, et al. Journal of molecular and cellular cardiology, 2015, 85: 131-139.
In vivo real-time visualization of mesenchymal stem cells tropism for cutaneous regeneration using NIR-II fluorescence imaging.
Chen G, Tian F, Li C, et al. Biomaterials, 2015, 53: 265-273.