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CREB-1 (phospho Ser142) Polyclonal Antibody

CREB-1 (phospho Ser142) Polyclonal Antibody

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Specification

Product name CREB-1 (phospho Ser142) Polyclonal Antibody
Immunogen Synthesized peptide derived from human CREB-1 around the phosphorylation site of S142
Host Rabbit
Reactivity Human, Mouse, Rat
Applications ELISA, IF, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:40000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative CREB1; Cyclic AMP-responsive element-binding protein 1; CREB-1; cAMP-responsive element-binding protein 1

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background CREB1 encodes a transcription factor (cAMP responsive element binding protein 1) that is a member of the leucine zipper family of DNA binding proteins. cAMP responsive element binding protein 1 binds as a homodimer to the cAMP-responsive element, an octameric palindrome. cAMP responsive element binding protein 1 is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of CREB1 results in several transcript variants encoding different isoforms.
Gene ID 1385
Alternative CREB1; Cyclic AMP-responsive element-binding protein 1; CREB-1; cAMP-responsive element-binding protein 1
Others Phospho-CREB-1 (S142) Polyclonal Antibody detects endogenous levels of CREB-1 protein only when phosphorylated at S142.
Accession P16220
Observed Band(KD) 37

Image & description

Fig.1. Western Blot analysis of various cells using Phospho-CREB-1 (S142) Polyclonal Antibody diluted at 1:500.

Fig.1. Western Blot analysis of various cells using Phospho-CREB-1 (S142) Polyclonal Antibody diluted at 1:500.

Fig.2. Western Blot analysis of HeLa cells using Phospho-CREB-1 (S142) Polyclonal Antibody diluted at 1:500.

Fig.2. Western Blot analysis of HeLa cells using Phospho-CREB-1 (S142) Polyclonal Antibody diluted at 1:500.

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