| Product name | Creatine Kinase M Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from the N-terminal region of human Creatine Kinase M at AA range: 10-90 |
| Host | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC 1:100-1:300;IF 1:200-1:1000;ELISA 1:40000;Not yet tested in other applications. |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | CKM; CKMM; Creatine kinase M-type; Creatine kinase M chain; M-CK |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 43kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Creatine kinase, M-typeencoded by CKM is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. |
| Gene ID | 1158 |
| Alternative | CKM; CKMM; Creatine kinase M-type; Creatine kinase M chain; M-CK |
| Others | Creatine Kinase M Polyclonal Antibody detects endogenous levels of Creatine Kinase M protein. |
| Accession | P06732 |
| Observed Band(KD) | 43 |
Fig.1. Western Blot analysis of various cells using Creatine Kinase M Polyclonal Antibody.
Fig.2. Western Blot analysis of Jurkat cells using Creatine Kinase M Polyclonal Antibody.
Fig.3. The picture was kindly provided by our customer.
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