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Collagen IV Polyclonal Antibody

Collagen IV Polyclonal Antibody

Views(459) Publications(0) Catalog no(ABP57493)
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Specification

Product name Collagen IV Polyclonal Antibody
Immunogen Synthesized peptide derived from Collagen IV at AA range: 1428-1443
Host Rabbit
Reactivity Human, Mouse, Rat
Applications ELISA, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:10000-1:20000).
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative collagen, type IV, alpha 1

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background COL4A1 (collagen type IV alpha 1 chain) encodes a type IV collagen alpha protein. Type IV collagen proteins are integral components of basement membranes. COL4A1 shares a bidirectional promoter with a paralogous gene on the opposite strand. The protein consists of an amino-terminal 7S domain, a triple-helix forming collagenous domain, and a carboxy-terminal non-collagenous domain. It functions as part of a heterotrimer and interacts with other extracellular matrix components such as perlecans, proteoglycans, and laminins. In addition, proteolytic cleavage of the non-collagenous carboxy-terminal domain results in a biologically active fragment known as arresten, which has anti-angiogenic and tumor suppressor properties. Mutations in COL4A1 cause porencephaly, cerebrovascular disease, and renal and muscular defects. Alternative splicing results in multiple transcript variants.
Gene ID 1282
Alternative collagen, type IV, alpha 1
Others Collagen IV Polyclonal Antibody detects endogenous levels of Collagen IV.
Accession P02462
Observed Band(KD) 130

Image & description

Fig. Western Blot analysis of Hela MCF-7 U2OS 293T KB 293T VEC cells using Collagen IV Polyclonal Antibody diluted at 1:800. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig. Western Blot analysis of Hela MCF-7 U2OS 293T KB 293T VEC cells using Collagen IV Polyclonal Antibody diluted at 1:800. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

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