| Product name | COL5A3 Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from the N-terminal region of human COL5A3 at AA range: 190-270 |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC 1:100-1:300;IF 1:200-1:1000;ELISA 1:20000;Not yet tested in other applications. |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | COL5A3; Collagen alpha-3(V) chain |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 170kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | COL5A3 encodes an alpha chain for one of the low abundance fibrillar collagens. Fibrillar collagen molecules are trimers that can be composed of one or more types of alpha chains. Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen. This gene product is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations. Mutations in COL5A3 are thought to be responsible for the symptoms of a subset of patients with Ehlers-Danlos syndrome type III. Messages of several sizes can be detected in northern blots but sequence information cannot confirm the identity of the shorter messages. |
| Gene ID | 50509 |
| Alternative | COL5A3; Collagen alpha-3(V) chain |
| Others | COL5A3 Polyclonal Antibody detects endogenous levels of COL5A3 protein. |
| Accession | P25940 |
| Observed Band(KD) | 170 |
Fig.1. Western Blot analysis of various cells using COL5A3 Polyclonal Antibody.
Fig.2. Western Blot analysis of K562 cells using COL5A3 Polyclonal Antibody.
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