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COL5A2 Polyclonal Antibody

COL5A2 Polyclonal Antibody

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Specification

Product name COL5A2 Polyclonal Antibody
Immunogen Synthesized peptide derived from the N-terminal region of human COL5A2
Host Rabbit
Reactivity Human
Applications ELISA, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative COL5A2; Collagen alpha-2(V) chain

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background COL5A2 encodes an alpha chain for one of the low abundance fibrillar collagens. Fibrillar collagen molecules are trimers that can be composed of one or more types of alpha chains. Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen. This gene product is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations. Mutations in this gene are associated with Ehlers-Danlos syndrome, types I and II.
Gene ID 1290
Alternative COL5A2; Collagen alpha-2(V) chain
Others COL5A2 Polyclonal Antibody detects endogenous levels of COL5A2 protein.
Accession P05997
Observed Band(KD) 145

Image & description

Fig.1. Western Blot analysis of HepG2 cells using COL5A2 Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.1. Western Blot analysis of HepG2 cells using COL5A2 Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.2. Immunohistochemical analysis of paraffin-embedded human-pancreas, antibody was diluted at 1:100.

Fig.2. Immunohistochemical analysis of paraffin-embedded human-pancreas, antibody was diluted at 1:100.

Fig.3. Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:100.

Fig.3. Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:100.

Fig.4. Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:100.

Fig.4. Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:100.

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