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COL4A2 Polyclonal Antibody

COL4A2 Polyclonal Antibody

Views(54) Publications(0) Catalog no(ABP53737)
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Specification

Product name COL4A2 Polyclonal Antibody
Immunogen Synthesized peptide derived from the N-terminal region of human COL4A2 at AA rangle: 120-200
Host Rabbit
Reactivity Human, Mouse
Applications ELISA, IF, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:20000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative COL4A2; Collagen alpha-2(IV) chain

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background COL4A2 encodes one of the six subunits of type IV collagen, the major structural component of basement membranes. The C-terminal portion of the protein, known as canstatin, is an inhibitor of angiogenesis and tumor growth. Like the other members of the type IV collagen gene family, COL4A2 is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter.
Gene ID 1284
Alternative COL4A2; Collagen alpha-2(IV) chain
Others COL4A2 Polyclonal Antibody detects endogenous levels of COL4A2 protein.
Accession P08572
Observed Band(KD) 150

Image & description

Fig.1. Western Blot analysis of SH-SY5Y cells using COL4A2 Polyclonal Antibody diluted at 1:2000.

Fig.1. Western Blot analysis of SH-SY5Y cells using COL4A2 Polyclonal Antibody diluted at 1:2000.

Fig.2. Western Blot analysis of SH-SY5Y 293T NIH-3T3 COLO cells using COL4A2 Polyclonal Antibody diluted at 1:2000.

Fig.2. Western Blot analysis of SH-SY5Y 293T NIH-3T3 COLO cells using COL4A2 Polyclonal Antibody diluted at 1:2000.

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