| Product name | Click EdUTP TUNEL Apoptosis Assay Kit (Green Fluorescence) |
| Applications notes | The Click EdUTP TUNEL detection method is a technique that significantly enhances the sensitivity by modifying the nucleotide analog EdUTP based on the basic principle of the conventional TUNEL (deoxyribonucleotide terminal transferase-mediated nick end labeling method). Its fundamental principle is derived from the classic TUNEL detection method. During apoptosis, the chromosomal DNA undergoes double-strand or single-strand breaks, generating a large number of 3'-hydroxyl (3'-OH) ends with a sticky end. Under the catalysis of deoxyribonucleotide terminal transferase (TdT), these 3'-OH ends can be labeled. The traditional TUNEL technique usually uses fluorescently labeled or biotinylated deoxyuridine triphosphate nucleotides (dUTP), and incorporates them into the DNA break sites through a one-step method by TdT enzyme, thereby achieving staining of apoptotic cells. Since normal or proliferating cells lack DNA breaks and 3'-OH formation, they are rarely stained, which makes the TUNEL technique a classic method for detecting cell apoptosis. |
| SampleType | Monolayer cells, suspension cells, paraffin sections, frozen sections |
| Kit components | • BSA Wash Solution(5×) •TritonX-100(100%) • DNase I(5 U/µL) •10×DNase I Buffer •TdT Enzyme •Equilibration Buffer •EdUTP •Probe Diluent •Reaction buffer(10×) •AbFluor 488 azide •Copper Reagent •Copper Protectant •Reducing Agent •DAPI(500×) •Tissue Autofluorescence Quencher •Proteinase K(20mg/mL) |
| Features & Benefits | 1. Utilizing the modified EdUTP nucleotide, the molecular size is small, and the efficiency of TdT enzyme incorporation is high, with a sensitivity superior to the traditional TUNEL method. 2. The click chemical reaction is rapid, efficient, highly specific, has a low background, and stable signal. 3. It is applicable to various sample types and compatible with fluorescence microscopes and flow cytometers. 4. A complete set of reagent components and optimized experimental protocols are provided. |
| Storage instructions | Stored at -20℃ for 12 months, protected from light |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | The Click EdUTP TUNEL detection method is a technique that significantly enhances the sensitivity by modifying the nucleotide analog EdUTP based on the basic principle of the conventional TUNEL (deoxyribonucleotide terminal transferase-mediated nick end labeling method). Its fundamental principle is derived from the classic TUNEL detection method. During apoptosis, the chromosomal DNA undergoes double-strand or single-strand breaks, generating a large number of 3'-hydroxyl (3'-OH) ends with a sticky end. Under the catalysis of deoxyribonucleotide terminal transferase (TdT), these 3'-OH ends can be labeled. The traditional TUNEL technique usually uses fluorescently labeled or biotinylated deoxyuridine triphosphate nucleotides (dUTP), and incorporates them into the DNA break sites through a one-step method by TdT enzyme, thereby achieving staining of apoptotic cells. Since normal or proliferating cells lack DNA breaks and 3'-OH formation, they are rarely stained, which makes the TUNEL technique a classic method for detecting cell apoptosis. |
Fig. Click EdUTP TUNEL Apoptosis Assay Kit (Green Fluorescence).
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