| Product name | CheKine™ Sulfide Content Assay Kit |
| SampleType | Water Sample |
| Alternative | Malic Acid |
| Kit components | •Reagent Ⅰ •Reagent Ⅱ •Reagent Ⅲ |
| Features & Benefits | The verified samples are complete in type and easy to operate. |
| Calibration range | 0.003125-0.5 mM |
| Limit of detection | 0.003125 mM |
| Usage notes | • If not assayed immediately, samples can be stored at -80°C for one month.• It is recommended to perform several dilutions of your sample to ensure the readings are within the standard value range.• Fresh samples are necessary for good results. |
| Storage instructions | Storage at 4°C upon receipt. Kit has a storage time of 12 months from receipt. Refer to list of materials supplied for storage conditions of individual components. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Sulfides exert significant effects on both organisms and aquatic environments, making the accurate detection of their concentrations of broad importance. The detection principle of this kit is based on the classic methylene blue colorimetric method. This method relies on the specific chromogenic reaction of sulfides: under strong acidic conditions containing Fe3+, sulfides (including H2S, HS+, and S2-, all of which are converted to H2S upon acidification) undergo a quantitative reaction with the chromogenic agent N,N-dimethyl-p-phenylenediamine to produce methylene blue, which exhibits a characteristic blue color. Methylene blue has its maximum absorption peak at 680 nm, and the absorbance at this wavelength shows a good linear relationship with the concentration of the chromogenic product. By comparison with a standard curve, the total sulfide content in the sample can be determined. The methylene blue method, with high sensitivity and good selectivity, is a classic standard method for the detection of sulfides in water. |
| Alternative | Malic Acid |
Fig.CheKine™ Sulfide Content Assay Kit
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