| Product name | CheKine™ Pro Cell Ferrous Ion (Fe²⁺) Fluorometric Assay Kit |
| SampleType | Cell |
| Alternative | Ferrous Ion (Fe²⁺) |
| Kit components | •Assay Buffer •RhoNox-4 (1000X) •Hoechst 33342 (1000X) |
| Features & Benefits | The verified samples are complete in type and easy to operate. |
| Storage instructions | Storage at -20°C upon receipt. Kit has a storage time of 12 months from receipt. Refer to list of materials supplied for storage conditions of individual components. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | There is virtually no truly free, hydrated Fe 2+ inside cells, the vast majority of iron is firmly bound to proteins (such as ferritin, heme, and iron-sulfur clusters). When we refer to intracellular Fe 2+ concentration, we are typically referring to that small fraction of Fe 2+ in the Labile Iron Pool (LIP) that is accessible to chelating agents and is redox-active. Precise regulation of intracellular iron homeostasis is critical for cell survival, and an imbalance in iron concentration can lead to the development of various diseases. When iron concentration is too high, ferrous ions can catalyze the production of hydroxyl radicals via the Fenton reaction, which in turn triggers lipid peroxidation, protein denaturation, and DNA damage, all of which are closely associated with major diseases such as cancer and Alzheimer’s disease. Conversely, when iron concentration is too low, heme synthesis is impeded, leading to iron-deficiency anemia and resulting in clinical symptoms such as tissue hypoxia and impaired immunity. This kit utilizes the red fluorescent probe RhoNox-4 to rapidly and sensitively detect Fe 2+ in live cells. RhoNox-4 exhibits high selectivity and sensitivity, binding specifically to ferrous ions without interference from common metal ions such as calcium, magnesium, zinc, and copper. This probe also features excellent cell membrane permeability, low cytotoxicity, and stability, making it suitable for real-time fluorescent imaging of ferrous ions in live cells, as well as for long-term monitoring and high-throughput assays. Upon binding to ferrous ions, its fluorescent signal is significantly enhanced, enabling precise detection of free ferrous ions within live cells. RhoNox-4 is suitable for research on cellular iron metabolism, oxidative stress, and ferroptosis. After binding to ferrous ions within cells, RhoNox-4 has a maximum excitation wavelength of 545 nm and an emission wavelength of 580 nm. |
| Alternative | Ferrous Ion (Fe²⁺) |
Fig.CheKine™ Pro Cell Ferrous Ion (Fe²⁺) Fluorometric Assay Kit
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