| Product name | CheKine™ Plus Micro Mitochondrial complex V Activity Assay Kit |
| Applications notes | Abbkine CheKine™ Micro Mitochondrial complex Ⅴ Activity Detection Kitis specially developed for the detection of complex I activity. The operation is simple and convenient, and the detection is more sensitive and accurate. complex Ⅴ hydrolyzes ATP to produce ADP and Pi. The activity of complex Ⅴ is determined by measuring Pi increase rate. |
| SampleType | :Animal and Plant Tissues,Cells |
| Kit components | • ReagentⅠ • Reagent Ⅱ • Reagent Ⅲ • Reagent Ⅳ • Reagent Ⅴ • Reagent Ⅵ • Reagent Ⅶ • Standard |
| Features & Benefits | • Detect the enzyme activity of complex V in plant tissues, animal tissues, cells and other samples. • Provides detailed sample preparation and results calculation methods. |
| Usage notes | • Before formal testing, it is recommended to select 2-3 samples with large expected differences for pre-experiment. •Microplate reader or ultraviolet spectrophotometer capable of measuring absorbance at 660 nm • Incubator, ice maker, refrigerated centrifuge • 96-well plate or microglass cuvette, precision pipettes, disposable pipette tips. •Deionized water • Homogenizer or mortar. |
| Storage instructions | The kit is valid for 12 months. Please refer to the table below Materials supplied and Storage conditions to store all the components. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Mitochondrial respiratory chain complex V, also known as F1F0-ATP synthase, is widely present in the mitochondria of animals, plants, microorganisms and cultured cells. Complex V consists of two subunits, F1 and F0. It could use the electrochemical gradient of protons produced by the respiratory chain to catalyze the synthesis of ATP, and also can hydrolyze ATP in a reversible process. In addition, complex V also exists in chloroplasts, heterotrophic bacteria and photosynthetic bacteria. Complex V is the key enzyme for mitochondrial oxidative phosphorylation and chloroplast photosynthetic phosphorylation to synthesize ATP. |
Fig.CheKine™ Micro Mitochondrial complex Ⅴ Activity Assay Kit
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