| Product name | CheKine™ Plus Micro Mitochondrial complex IV Activity Assay Kit |
| Applications notes | Abbkine CheKine™ Micro Mitochondrial complex Ⅴ Activity Detection Kitis specially developed for the detection of complex I activity. The operation is simple and convenient, and the detection is more sensitive and accurate. complex Ⅴ hydrolyzes ATP to produce ADP and Pi. The activity of complex Ⅴ is determined by measuring Pi increase rate. |
| Kit components | • ReagentⅠ • Reagent Ⅱ • Reagent Ⅲ • Reagent Ⅳ • Reagent Ⅴ |
| Features & Benefits | • Detect the enzyme activity of complex V in plant tissues, animal tissues, cells and other samples. • Provides detailed sample preparation and results calculation methods. |
| Usage notes | • Before formal testing, it is recommended to select 2-3 samples with large expected differences for pre-experiment. •Microplate reader or ultraviolet spectrophotometer capable of measuring absorbance at 550 nm • Incubator, ice maker, refrigerated centrifuge • 96-well plate or microglass cuvette, precision pipettes, disposable pipette tips. •Deionized water • Homogenizer or mortar. |
| Storage instructions | The kit is valid for 12 months. Please refer to the table below Materials supplied and Storage conditions to store all the components. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Mitochondrial respiratory chain complex Ⅳ (EC 7.1.1.9), also known as cytochrome C oxidase, is also a common component of the main and branch pathways of the mitochondrial respiratory chain. It is responsible for catalyzing the oxidation of reduced cytochrome C, and ultimately transferring electrons to oxygen to produce water. CheKine™ Slow Micro Mitochondrial Complex Ⅳ Activity Assay Kit provides a convenient tool for detection of Mitochondrial complex Ⅳ Activity. The principle is Reduced cytochrome C has characteristic light absorption at 550 nm. Mitochondrial respiratory chain complex Ⅳ could catalyze reduced cytochrome C to oxidized cytochrome C. Therefore, the decreasing rate of light absorption at 550 nm can reflect the enzyme activity of mitochondrial respiratory chain complex Ⅳ. It can be used to determine animal, plant tissues and cell samples |
Fig.CheKine™ Micro Mitochondrial complex Ⅳ Activity Assay Kit
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