| Product name | CheKine™ Plus Micro Mitochondrial complex III Activity Assay Kit |
| Applications notes | Abbkine CheKine™ Micro Mitochondrial Complex Ⅲ Activity Detection Kitis specially developed for the detection of complex I activity. The operation is simple and convenient, and the detection is more sensitive and accurate. Complex III could transfer the hydrogen from reduced CoQ to cytochrome C to produce reduced cytochrome C. Unlike oxidized cytochrome C, reduced cytochrome C has characteristic light absorption at 550 nm. So, the increasing of light absorption rate can reflect the activity of Complex III enzymes, and Abbkine CheKine™ Micro Mitochondrial complex Ⅲ Activity Assay Kit can detect animal and plant tissues and cell samples. |
| Kit components | • ReagentⅠ • Reagent Ⅱ • Reagent Ⅲ • Reagent Ⅳ • Reagent Ⅴ • Reagent Ⅵ |
| Features & Benefits | • Detect the enzyme activity of complex III in plant tissues, animal tissues, and cell samples. • Provides detailed sample preparation and results calculation methods. |
| Usage notes | • Before formal testing, it is recommended to select 2-3 samples with large expected differences for pre-experiment. •Microplate reader or ultraviolet spectrophotometer capable of measuring absorbance at 550 nm • Incubator, ice maker, refrigerated centrifuge • 96-well plate or microglass cuvette, precision pipettes, disposable pipette tips. •Deionized water • Homogenizer or mortar. |
| Storage instructions | The kit is valid for 12 months. Please refer to the table below Materials supplied and Storage conditions to store all the components. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Mitochondrial respiratory chain complex III (EC 1.10.2.2), also known as CoQ-cytochrome C reductase, is widely present in the mitochondria of animals, plants, microorganisms and cultured cells. It plays a key role at the main and branch pathways in the mitochondrial respiratory electron transport chain. Complex III could transfer the hydrogen of the reduced CoQ to cytochrome C ,and produce reduced cytochrome C. |
Fig.CheKine™ Plus Micro Mitochondrial complex III Activity Assay Kit
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