| Product name | CheKine™ Plus Micro Mitochondrial complex II Activity Assay Kit |
| Applications notes | Abbkine CheKine™ Micro Mitochondrial Complex II Activity Detection Kitis specially developed for the detection of complex I activity. The operation is simple and convenient, and the detection is more sensitive and accurate.The reduced coenzyme Q, which is the catalytic product of the mitochondrial respiratory chain complex II, can further reduce 2,6-dichloroindoxyl, which has a characteristic absorption peak at 605 nm. So, the activity of complex II could be calculated according to the decrease rate of 2,6-dichloroindoxyl. |
| Kit components | • ReagentⅠ • Reagent Ⅱ • Reagent Ⅲ • Reagent Ⅳ |
| Features & Benefits | • Detect the enzyme activity of complex II in plant tissues, animal tissues, and cells. • Provides detailed sample preparation and results calculation methods. |
| Usage notes | • Before formal testing, it is recommended to select 2-3 samples with large expected differences for pre-experiment. •Microplate reader or ultraviolet spectrophotometer capable of measuring absorbance at 600 nm • Incubator, ice maker, refrigerated centrifuge • 96-well plate or microglass cuvette, precision pipettes, disposable pipette tips. •Deionized water • Homogenizer or mortar. |
| Storage instructions | The kit is valid for 12 months. Please refer to the table below Materials supplied and Storage conditions to store all the components. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Mitochondrial complex II, also known as succinic acid-coenzyme Q reductase, is widely found in mitochondria of animals, plants, microorganisms and cultured cells. Complex II could catalyze the oxidation of succinic acid to produce fumaric acid, while the cofactor FAD reduced to FADH2,which further reduces oxidized coenzyme Q to produce reduced coenzyme Q, which is a branch of the respiratory electron transport chain. |
Fig.CheKine™ Plus Micro Mitochondrial complex II Activity Assay Kit
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