| Product name | CheKine™ Plus Micro Mitochondrial complex I Activity Assay Kit |
| Applications notes | Abbkine CheKine™ Micro Mitochondrial Complex I Activity Detection Kitis specially developed for the detection of complex I activity. The operation is simple and convenient, and the detection is more sensitive and accurate. Complex I can catalyze the dehydrogenation of NADH to NAD+. The oxidation rate of NADH can be determined at 340 nm to calculate the activity of Complex I . |
| Kit components | • ReagentⅠ • Reagent Ⅱ • Reagent Ⅲ • Reagent Ⅳ • Reagent Ⅴ |
| Features & Benefits | • Detect the enzyme activity of complex Ⅰ in plant tissues, animal tissues, cells. • Provides detailed sample preparation and results calculation methods |
| Usage notes | • Before formal testing, it is recommended to select 2-3 samples with large expected differences for pre-experiment. •Microplate reader or ultraviolet spectrophotometer capable of measuring absorbance at 340 nm • Incubator, ice maker, refrigerated centrifuge • 96-well UV plate or microquartz cuvette, precision pipettes, disposable pipette tips. • Homogenizer or mortar. |
| Storage instructions | The kit is valid for 12 months. Please refer to the table below Materials supplied and Storage conditions to store all the components. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Mitochondrial respiratory chain complex I （EC 1.6.5. 3）, also known as NADH-CoQ reductase or NADH dehydrogenase ,is the largest protein complex in the mitochondrial intima. Complex I is widely found in the mitochondria of animals, plants, microorganisms and cultured cells. This enzyme catalyzes the transfer of a pair of electrons from NADH to CoQ, and at the same time it can reduce O2 to produce O2-, which is the main part of the respiration electron transport chain to produce O2-. The activity of Complex I can not only reflect the status of responsibility electron transfer chain (ETC), but also reflect the production of reactive oxygen species (ROS) . |
Fig.CheKine™ Plus Micro Mitochondrial complex I Activity Assay Kit
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