| Product name | CheKine™ Micro Soil Neutral Xylanase (S-NEX) Activity Assay Kit |
| SampleType | Soil |
| Alternative | Soil Neutral Xylanase (S-NEX) |
| Kit components | • Reagent I •Reagent Ⅱ •Reagent Ⅲ •Standard |
| Features & Benefits | The verified samples are complete in type and easy to operate． |
| Usage notes | • If not assayed immediately, samples can be stored at -80°C for one month.• It is recommended to perform several dilutions of your sample to ensure the readings are within the standard value range.• Fresh samples are necessary for good results. If don't perform the assay at the same time, it's better to complete the Sample Preparation step before storing the samples. |
| Storage instructions | Storage at 4°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Xylanase (EC 3.2.1.8) is primarily produced by microorganisms and can catalyze the hydrolysis of xylan, also known as pentosanase or hemicellulase. It breaks down the cell walls of raw materials in brewing or feed industries and degrades β-glucans, reducing the viscosity of materials during brewing, promoting the release of effective substances, and decreasing non-starch polysaccharides in feed to enhance nutrient absorption. Therefore, it is widely used in brewing and feed industries. NEX (Neutral Xylanase) is generally isolated from microorganisms with an optimal growth pH range of 6-8. CheKine™ Micro Soil Neutral Xylanase (S-NEX) Activity Assay Kit provides a simple, convenient, and rapid method for detecting neutral xylanase activity in soil samples. The principle of this assay is that NEX catalyzes the degradation of xylan into reducing oligosaccharides and monosaccharides under neutral conditions. These products further react with 3,5-dinitrosalicylic acid (DNS) in a boiling water bath, resulting in a colorimetric reaction with a characteristic absorption peak at 540 nm. The intensity of the reaction mixture's color is proportional to the amount of reducing sugars produced by enzymatic hydrolysis. By measuring the increase in absorbance at 540 nm, the activity of S-NEX can be calculated. |
| Alternative | Soil Neutral Xylanase (S-NEX) |
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