| Product name | CheKine™ Micro Ribulose Bisphosphate Carboxylase/Oxygenase (Rubisco) Assay Kit |
| Applications notes | CheKine™ Micro Ribulose bisphosphate carboxylase/oxygenase (Rubisco) Assay Kit provides a simple method for detecting Rubisco activity in plant tissues. One molecule of ribulose-1,5-diphosphate (RuBP) combines with one molecule of CO2 to produce two molecules of 3-phosphoglyceric acid (PGA). Glyceraldehyde-3-phosphate can be produced by PGA through the action of additional 3-phosphoglycerate kinase and glyceraldehyde-3-phosphate dehydrogenase, which is accompanied by NADH oxidation to produce NAD+. NADH has a characteristic absorption peak at 340 nm, while NAD+ does not. Rubisco activity is calculated by measuring the rate of decrease in light absorption at 340 nm. |
| Alternative | Rubisco; Bisphosphate; Carboxylase/Oxygenase |
| Kit components | • Extraction BufferⅠ • Extraction BufferⅡ • ReagentⅠ • ReagentⅡ • ReagentⅢ • ReagentⅣ |
| Features & Benefits | • Determination of Rubisco activity in plant tissues. • Detailed sample preparation and result calculation methods are provided. |
| Storage instructions | Storage at -20°C and protected from light upon receipt. Kit has a storage time of 6 months form receipt. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | 1, 5-ribulose bisphosphate carboxylase/oxygenase (Rubisco, EC 4.1.1.39) is a key enzyme in plant photosynthesis, which not only controls the fixation of CO2, but also controls the distribution of carbon to the Calvin cycle and the photorespiration cycle. Its activity directly affects the photosynthetic rate. |
| Alternative | Rubisco; Bisphosphate; Carboxylase/Oxygenase |
Fig. CheKine™ Micro Ribulose Bisphosphate Carboxylase/Oxygenase (Rubisco) Assay Kit.
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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