| Product name | CheKine™ Micro Phosphoenolpyruvate (PEP) Content Assay Kit |
| SampleType | Animal and Plant tissues, Cells, Plasma, Serum or other Liquid samples |
| Alternative | Phosphoenolpyruvate (PEP) Content |
| Kit components | •Extraction Buffer •Reagent Ⅰ •Reagent Ⅱ •Reagent Ⅲ •Reagent Ⅳ •Reagent Ⅴ •Standard |
| Features & Benefits | The verified samples are complete in type and easy to operate. |
| Calibration range | 10-1000 nmol/mL |
| Limit of detection | 10 nmol/mL |
| Usage notes | • If not assayed immediately, samples can be stored at -80°C for one month.• It is recommended to perform several dilutions of your sample to ensure the readings are within the standard value range.• Fresh samples are necessary for good results. |
| Storage instructions | Storage at 4°C upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Phosphoenolpyruvate (PEP) is a key metabolic intermediate in living organisms. It plays an indispensable role in crucial metabolic pathways such as glycolysis, gluconeogenesis, and the synthesis of aromatic amino acids, being of great significance for maintaining the balance of cellular energy metabolism and material synthesis. CheKine™Micro Phosphoenolpyruvate (PEP) Content Assay Kit can be used to detect biological samples such as animal and plant tissues, cells, plasma, serum or other liquid samples. In the sample, PEP undergoes a transphosphorylation reaction with adenosine diphosphate (ADP) under the catalysis of pyruvate kinase (PK), generating pyruvate and adenosine triphosphate (ATP). Subsequently, pyruvate is oxidized under the action of pyruvate oxidase, accompanied by the reduction of flavin adenine dinucleotide (FAD), producing reduced flavin adenine dinucleotide (FADH2). The generated FADH2 is further oxidized to regenerate FAD and release hydrogen peroxide (H2O2). Finally, in the catalytic system of peroxidase, H2O2 undergoes a coupled color reaction with 4-aminoantipyrine (4-AAP) and N-ethyl-N-(2-hydroxy-3-propanesulfonyl)-3-methylaniline (TOOS), generating stable red quinone imine compounds. By detecting the absorbance value at 554 nm, the content of PEP in the sample can be calculated. |
| Alternative | Phosphoenolpyruvate (PEP) Content |
Fig.CheKine™ Micro Phosphoenolpyruvate (PEP) Content Assay Kit
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