| Product name | CheKine™ Micro Hexokinase (HK) Activity Assay Kit |
| Applications notes | CheKine™ Micro Hexokinase (HK) Activity Assay Kit provides a simple, convenient, rapid and colorimetric HK detection method.HK catalyzes glucose synthesis of 6-phosphoglucose, and 6-phosphoglucose dehydrogenase further catalyzes 6-phosphoglucose dehydrogenation to NADH, which reduces WST-8 to orange formazan, with the maximum absorption peak detected at around 450 nm. The enzyme activity was calculated by detecting the increase rate of light absorption at 450 nm |
| Kit components | •Extraction Buffer • Assay Buffer • Substrate Mix • Enzyme Mix • WST-8 • Enhancer • NADH Standard |
| Features & Benefits | • Detect hexokinase (HK) activity in bacterial supernatant of serum (plasma) animal and plant tissues/cells • Detailed sample preparation and results calculation methods are provided |
| Calibration range | 31.25-2000μM |
| Usage notes | • Do not mix or substitute reagents or materials from other kit lots or vendors. • It is advisable to measure serum potassium as soon as possible after blood collection. • Ensure all reagents and equipment are at the appropriate temperature before starting the assay. |
| Storage instructions | Storage at -20°C and protected from light upon receipt. Kit has a storage time of 12 months form receipt. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Hexokinase (HK) is widely found in animals, plants, microorganisms and cultured cells. It is the first key enzyme in the process of glucose decomposition, catalyzing the conversion of glucose to glucose 6-phosphoglucose, which is the intersection of glycolysis and pentose phosphate pathways |
Fig.CheKine™ Micro Hexokinase(HK) Assay Kit
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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