| Product name | CheKine™ Micro Glutathione Peroxidase (GSH-Px) Activity Assay Kit |
| Applications notes | GSH-Px catalyzes H2O2 to oxidize GSH to produce GSSG; glutathione reductase (GR) catalyzes NADPH to reduce GSSG to regenerate GSH, while NADPH oxidizes to produce NADP+; NADPH has a characteristic absorption peak at 340 nm, while NADP+ does not; NADPH dehydrogenation rate is determined by measuring the decrease rate of absorbance at 340 nm to calculate GSH-Px activity. |
| Kit components | • Assay Buffer • Substrate • GR • H2O2 |
| Features & Benefits | • Determination of GSH-Px activity in serum, plasma, plant tissue/cell lysates and other biological fluids. |
| Usage notes | • 96 Well Clear Flat Bottom UV-Transparent Microplate is needed. • The whole processes need to be carried out on ice, and the enzyme activity should be determined on the same day, to avoid repeated freeze-thaw of the homogenate solution. • Prepare mixed reagents for use, and place on ice. • The measuring process should be operated quickly and only 1 ~ 2 blank well should be needed. • The reaction temperature has influence on the result. Keep the temperature at 25°C (for general species) or 37°C (for mammals). |
| Storage instructions | Storage at -20°C and Keep from light immediately upon receipt. Kit has a storage time of 12 months from receipt. Refer to list of materials supplied for storage conditions of individual components. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | GSH-Px is one of the main enzymes that catalyze the oxidation of reduced glutathione (GSH) in the glutathione redox cycle. GSH-Px not only specifically catalyzes the reaction between reduced glutathione and ROS to form oxidized glutathione GSSG, thereby protecting the biofilm from ROS damage and maintaining the normal function of cells; it also protects the liver and improves the body immunity, antagonizing the harmful effects of harmful metal ions on the body and increasing the body's ability to resist radiation. |
Fig. CheKine™ Micro Glutathione Peroxidase (GSH-Px) Activity Assay Kit
Author：Roy JR, Janaki CS, Jayaraman S, et al. Publication name：Antioxidants IF：7.675
Author： Zhang M, Zhu D, Wan Y Publication name：ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS IF：4.114
Author：Jing Sun，Mingqiang Chen，Zhengyi Fu，Jingru Yang，Zhenhua Ma Publication name：ORIGINAL RESEARCH IF：3.661
Author：N Liu, S Zhou, OJ Olatunji, Y Wu Publication name：Arabian Journal of Chemistry IF：3.298
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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