| Product name | CheKine™ Micro Glutamine (Gln) Content Assay Kit |
| SampleType | Serum,Plasma,Animal/PlantTissues,Wholeblood,Cells,BacteriaandCellculturemedia |
| Alternative | Gln |
| Kit components | •Extraction Buffer •Assay Buffer •Enzyme A •Enzyme A diluent •Enzyme B •NAD •WST-8 •Enhancer •Glutamine Standard |
| Features & Benefits | The verified samples are complete in type and easy to operate． |
| Usage notes | • If not assayed immediately, samples can be stored at -80°C for one month.• It is recommended to perform several dilutions of your sample to ensure the readings are within the standard value range.• Fresh samples are necessary for good results. If don't perform the assay at the same time, it's better to complete the Sample Preparation step before storing the samples. |
| Storage instructions | Storedat-20℃for6months,protectedfromlight |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Glutamine(Gln)istheγ-carboxylamidateofL-glutamicacid.Itisanabundantaminoacidinorganismsandiscalleda conditionallyessentialaminoacid.Glutamineisanimportantcarrierofnitrogenflowinthebody.Itisnotonlyanimportant substrateforproteinsynthesis,butalsoanimportanthubconnectingaminoacidmetabolismandsugarmetabolism.Itplaysa regulatoryroleinvariousmetabolicactivitiesoforganisms. Thedetectionprincipleofthiskitistouseglutaminase(Glutaminase,GLS)tocatalyzeglutaminetogenerateglutamate,and glutamatedehydrogenase(Glutamatedehydrogenase,GLDH)tocatalyzeglutamateandNADtogenerateα-ketoglutarate,NADH andNH4+.Inthepresenceofelectronacceptors,NADHandWST-8generateorange-yellowformazan,andmeasuringits absorbancevalueat450nmcancalculatetheglutaminecontentinthesample. |
| Alternative | Gln |
Fig.CheKine™ Micro Glutamine (Gln) Content Assay Kit
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