| Product name | CheKine™ Micro Copper（Cu）Content Assay Kit |
| SampleType | Animal Tissues, Cells , Plasma, Serum |
| Alternative | Copper（Cu）Content |
| Kit components | •Reagent Ⅰ •Reagent Ⅱ •Reagent Ⅲ •Standard（5μmol/L） |
| Features & Benefits | The verified samples are complete in type and easy to operate. |
| Calibration range | 0.13 µM-5 µM |
| Limit of detection | 0.13 µM |
| Usage notes | • If not assayed immediately, samples can be stored at -80°C for one month.• It is recommended to perform several dilutions of your sample to ensure the readings are within the standard value range.• Fresh samples are necessary for good results. |
| Storage instructions | Storage at 4°C upon receipt. Kit has a storage time of 12 months from receipt. Refer to list of materials supplied for storage conditions of individual components. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Copper is an essential trace transition metal in eukaryotic cells, acting as a cofactor for key enzymes including cytochrome c oxidase and SOD1. Its intracellular homeostasis is tightly regulated and critical for mitochondrial respiration, antioxidant defense, neurotransmitter synthesis, and iron metabolism. Both copper deficiency and excess impair cellular function by inducing oxidative stress and disrupting mitochondrial metabolism. Additionally, copper participates in hematopoiesis by catalyzing hemoglobin synthesis, and supports skeletal and neural development. Thus, accurate quantification of total copper is fundamental to understanding copper homeostasis. This kit employs a specific colorimetric assay under acidic conditions, copper ions are fully released from copper-binding proteins (e.g., ceruloplasmin), and in a reducing environment, they form a stable colored complex with the highly selective chelator 3,5-DiBr-PAESA, with maximal absorbance at 575–585 nm. The absorbance correlates linearly with total copper concentration over a defined range. Quantification is achieved by measuring absorbance on a microplate reader against a standard curve. |
| Alternative | Copper（Cu）Content |
Fig.CheKine™ Micro Copper（Cu）Content Assay Kit
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