| Product name | CheKine™ Micro α-Ketoglutaric Acid(α-KG)Content Assay Kit |
| SampleType | Animal and Plant Tissues, Bacteria, Cells, Fungi, Plasma, Serum, Urine, Cell Culture |
| Alternative | α-Ketoglutarat |
| Kit components | •Extraction Buffer Ⅰ •Extraction Buffer Ⅱ •Reagent Ⅰ •Reagent Ⅱ •Reagent Ⅲ •Standard |
| Features & Benefits | The verified samples are complete in type and easy to operate． |
| Usage notes | • If not assayed immediately, samples can be stored at -80°C for one month.• It is recommended to perform several dilutions of your sample to ensure the readings are within the standard value range.• Fresh samples are necessary for good results. If don't perform the assay at the same time, it's better to complete the Sample Preparation step before storing the samples. |
| Storage instructions | Storage at 4°C and Keep from light immediately upon receipt. Kit has a storage time of 12 months from receipt. Refer to list of materials supplied for storage conditions of individual components. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | α-Ketoglutarate (α-KG), a crucial metabolic intermediate in the tricarboxylic acid cycle, serves as a key junction linking intracellular carbon and nitrogen metabolism. As a short-chain carboxylic acid molecule, α-KG is the precursor of multiple essential amino acids including glutamine and glutamate. It not only directly participates in energy supply but also engages in various intracellular chemical reactions, exerting a variety of physiological functions. CheKine™ Micro α-Ketoglutaric Acid (α-KG) Content Assay Kit can be used to detect biological samples such as animal and plant tissues, bacteria, cells, fungi, plasma, serum , urine, cell culture or other liquid samples. In the kit, glutamate dehydrogenase (GDH) catalyzes the reaction involving NH4+ , α-ketoglutarate, and NADH, resulting in the formation of glutamate and NAD+ , which causes a decrease in absorbance at 340 nm. The content of α-ketoglutarate is calculated by measuring the change in NADH. |
| Alternative | α-Ketoglutarat |
Fig.CheKine™ Micro α-Ketoglutaric Acid(α-KG)Content Assay Kit
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