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CheKine™ Micro α-Amylase Activity Assay Kit

CheKine™ Micro α-Amylase Activity Assay Kit

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Specification

Product name CheKine™ Micro α-Amylase Activity Assay Kit
Applications notes CheKine™ Micro α-Amylase Activity Assay Kit provides a convenient tool for detection of α-Amylase Activity. The principle is that starch hydrolase catalyzes the hydrolysis of starch to produce reducing sugar, which reduces 3, 5-dinitrosalicylic acid to produce brown-red substance with an absorption peak at 540 nm. Amylase activity was calculated by measuring the absorbance increase rate at 540 nm.

Product Properties

Kit components •DNS Reagent
•Substrate
•Standard
Features & Benefits • Determination of α-Amylase Activity in a variety of biological samples such as serum, plasma, animal and plant tissues.
• Detailed sample preparation and result calculation methods are provided.
• Standard and standard curve line are provided.
Calibration range 0.0156-1 mg/mL
Limit of detection 0.0078 mg/mL
Storage instructions Storage at 4°C and protected from light upon receipt. Kit has a storage time of 12 months form receipt.
Shipping Blue ice
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Starch hydrolases, including α -amylase (α -Al, EC 3.2.1.1) and β -amylase (β -Al). α -amylase randomly catalyzes the hydrolysis of α-1, 4-glycosidic bond in starch to generate glucose, maltose, maltose, dextrin and other reducing sugars, while reducing the viscosity of starch, so it is also called liquefaction enzyme.

Image & description

Fig. CheKine™ Micro α-Amylase Activity Assay Kit

Fig. CheKine™ Micro α-Amylase Activity Assay Kit

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