| Product name | CheKine™ Free Fatty Acid (FFA/NEFA) Content Assay Kit |
| SampleType | Serum (plasma), animal and plant tissues, cells |
| Alternative | Free Fatty Acid (FFA/NEFA) Content |
| Kit components | •Reagent Ⅰ •Reagent Ⅱ •Reagent Ⅲ •Standard |
| Features & Benefits | The verified samples are complete in type and easy to operate. |
| Usage notes | • If not assayed immediately, samples can be stored at -80°C for one month.• It is recommended to perform several dilutions of your sample to ensure the readings are within the standard value range.• Fresh samples are necessary for good results. |
| Storage instructions | Storage at -20°C upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Non-esterified fatty acids (NEFA) are the primary product of lipolysis in adipose tissue, and their serum levels directly reflect the body's lipid metabolic status. Elevated serum NEFA levels are closely associated with insulin resistance, typeⅡdiabetes, obesity, and cardiovascular diseases; conversely, reduced levels indicate impaired endocrine function. Therefore, NEFA testing is an important indicator for screening lipid metabolic disorders and monitoring disease progression. This assay kit employs an enzyme-coupled colorimetric method. Free fatty acids in the sample are converted into acetyl-CoA by acyl-CoA synthetase (ACS), which is then oxidized by acyl-CoA oxidase (ACOD) to produce hydrogen peroxide. Under catalysis by peroxidase (POD), hydrogen peroxide reacts with a chromogenic substrate to form a purple quinone dye. The absorbance of this dye at 546 nm is directly proportional to the concentration of free fatty acids in the sample. |
| Alternative | Free Fatty Acid (FFA/NEFA) Content |
Fig.CheKine™ Free Fatty Acid (FFA/NEFA) Content Assay Kit
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