| Product name | CheKine™ Aspartate Aminotransferase (AST/GOT) Activity Assay Kit |
| SampleType | Animal Tissues, Cells, Plasma, Serum |
| Alternative | Aspartate Aminotransferase (AST/GOT) Activity |
| Kit components | •Reagent Ⅰ •Reagent Ⅱ •Reagent Ⅲ •Reagent Ⅳ •Standard |
| Features & Benefits | The verified samples are complete in type and easy to operate. |
| Usage notes | • If not assayed immediately, samples can be stored at -80°C for one month.• It is recommended to perform several dilutions of your sample to ensure the readings are within the standard value range.• Fresh samples are necessary for good results. |
| Storage instructions | Storage at 4°C upon receipt. Kit has a storage time of 12 months from receipt. Refer to list of materials supplied for storage conditions of individual components. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Aspartate Aminotransferase (AST, formerly known as GOT) is an intracellular aminotransferase, widely distributed in the mitochondria and cytoplasm of animal/human tissues including myocardium, liver, skeletal muscle, kidney and others. Its concentration is extremely low in body fluids under physiological conditions; massive enzyme release into the blood occurs upon cellular injury and rupture. AST catalyzes the reversible transamination between L-aspartate and α-ketoglutarate, participating in in vivo amino acid metabolism and gluconeogenesis pathways. In clinical assays, AST activity serves as a critical biomarker for assessing the extent of hepatocyte and cardiomyocyte injury. CheKine™Aspartate Aminotransferase (AST/GOT) Activity Assay Kit (Reitman-Frankel Method) can be used to detect biological samples such as animal tissues, cells, plasma and serum. Under the conditions of 37 ℃ and pH 7.4, aspartate aminotransferase (AST/GOT) catalyzes the transamination reaction between α-ketoglutarate and L-aspartate to produce glutamate and pyruvate. Pyruvate reacts with 2,4-dinitrophenylhydrazine to form pyruvate phenylhydrazone, which exhibits a brownish-red color under alkaline conditions. The absorbance is measured at 505 nm, and the AST/GOT enzyme activity can be calculated accordingly. |
| Alternative | Aspartate Aminotransferase (AST/GOT) Activity |
Fig.CheKine™ Aspartate Aminotransferase (AST/GOT) Activity Assay Kit
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