| Product name | CheKine™ Alanine Aminotransferase (ALT/GPT) Activity Assay Kit |
| SampleType | Serum, Plasma, Animal Tissues, Cells |
| Alternative | Alanine Aminotransferase (ALT/GPT) Activity，ALT |
| Kit components | •Reagent Ⅰ •Reagent Ⅱ •Reagent Ⅲ •Reagent Ⅳ •Standard |
| Features & Benefits | The verified samples are complete in type and easy to operate. |
| Usage notes | • If not assayed immediately, samples can be stored at -80°C for one month.• It is recommended to perform several dilutions of your sample to ensure the readings are within the standard value range.• Fresh samples are necessary for good results. |
| Storage instructions | Storage at 4°C upon receipt. Kit has a storage time of 24 months from receipt. Refer to list of materials supplied for storage conditions of individual components. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Alanine Aminotransferase (ALT/GPT) is widely present in animals, plants, microorganisms and cultured cells. It catalyzes the reversible amino reaction and is an important enzyme for amino acid metabolism. Mammalian hepatocytes exhibit high glutamic-pyruvic transaminase (ALT) activity. When hepatocytes undergo necrosis, ALT is released into the bloodstream, resulting in a marked increase in serum ALT activity. Serum ALT activity level is an important biochemical indicator of liver damage, which can reflect the severity of liver damage. CheKine™Alanine Aminotransferase (ALT/GPT) Activity Assay Kit (Reitman-Frankel Method) provides a simple method for detecting ALT/GPT activity in a variety of biological samples such as Serum, Plasma, Animal Tissues, Cells. In the assay, ALT catalyzes the transamination reaction of α-ketoglutarate and alanine at 37℃and pH7.4 to produce glutamic acid and pyruvate; Pyruvate can react with 2,4-dinitrophenylhydrazine to form pyruvate phenylhydrazone, which appears brownish red under alkaline conditions and has a characteristic absorption peak at 505 nm. The rate of pyruvate phenylhydrazone increase at 505 nm can reflect ALT/GPT activity. |
| Alternative | Alanine Aminotransferase (ALT/GPT) Activity，ALT |
Fig.CheKine™ Alanine Aminotransferase (ALT/GPT) Activity Assay Kit
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