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Cerberus Polyclonal Antibody

Cerberus Polyclonal Antibody

Views(49) Publications(0) Catalog no(ABP53141)
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Specification

Product name Cerberus Polyclonal Antibody
Immunogen Synthesized peptide derived from the Internal region of human Cerberus
Host Rabbit
Reactivity Human, Rat
Applications ELISA, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative CER1; DAND4; Cerberus; Cerberus-related protein; DAN domain family member 4

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background CER1 encodes a cytokine member of the cysteine knot superfamily, characterized by nine conserved cysteines and a cysteine knot region. The cerberus-related cytokines, together with Dan and DRM/Gremlin, represent a group of bone morphogenetic protein (BMP) antagonists that can bind directly to BMPs and inhibit their activity.
Gene ID 9350
Alternative CER1; DAND4; Cerberus; Cerberus-related protein; DAN domain family member 4
Others Cerberus Polyclonal Antibody detects endogenous levels of Cerberus protein.
Accession O95813
Observed Band(KD) 30

Image & description

Fig.1. Western Blot analysis of MCF-7 cells using Cerberus Polyclonal Antibody. Antibody was diluted at 1:500. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.1. Western Blot analysis of MCF-7 cells using Cerberus Polyclonal Antibody. Antibody was diluted at 1:500. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.2. Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:100.

Fig.2. Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:100.

Fig.3. Immunohistochemical analysis of paraffin-embedded human-spleen, antibody was diluted at 1:100.

Fig.3. Immunohistochemical analysis of paraffin-embedded human-spleen, antibody was diluted at 1:100.

Fig.4. Western Blot analysis of Rat-heart Rat-brain using Cerberus Polyclonal Antibody diluted at 1:500. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.4. Western Blot analysis of Rat-heart Rat-brain using Cerberus Polyclonal Antibody diluted at 1:500. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

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