| Product name | Cerberus Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from the Internal region of human Cerberus |
| Host | Rabbit |
| Reactivity | Human,Rat |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC: 1:100-1:300;ELISA 1:20000;IF 1:50-200 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | CER1; DAND4; Cerberus; Cerberus-related protein; DAN domain family member 4 |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 30kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | CER1 encodes a cytokine member of the cysteine knot superfamily, characterized by nine conserved cysteines and a cysteine knot region. The cerberus-related cytokines, together with Dan and DRM/Gremlin, represent a group of bone morphogenetic protein (BMP) antagonists that can bind directly to BMPs and inhibit their activity. |
| Gene ID | 9350 |
| Alternative | CER1; DAND4; Cerberus; Cerberus-related protein; DAN domain family member 4 |
| Others | Cerberus Polyclonal Antibody detects endogenous levels of Cerberus protein. |
| Accession | O95813 |
| Observed Band(KD) | 30 |
Fig.1. Western Blot analysis of MCF-7 cells using Cerberus Polyclonal Antibody. Antibody was diluted at 1:500. Secondary antibody (catalog#: A21020) was diluted at 1:20000.
Fig.2. Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:100.
Fig.3. Immunohistochemical analysis of paraffin-embedded human-spleen, antibody was diluted at 1:100.
Fig.4. Western Blot analysis of Rat-heart Rat-brain using Cerberus Polyclonal Antibody diluted at 1:500. Secondary antibody (catalog#: A21020) was diluted at 1:20000.
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