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Cdk4 Polyclonal Antibody

Cdk4 Polyclonal Antibody

Views(27) Publications(0) Catalog no(ABP52929)
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Specification

Product name Cdk4 Polyclonal Antibody
Immunogen Synthesized peptide derived from the N-terminal region of human Cdk4
Host Rabbit
Reactivity Human, Mouse, Rat
Applications ELISA, IF, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative CDK4; Cyclin-dependent kinase 4; Cell division protein kinase 4; PSK-J3

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Cyclin-dependent kinase 4 encoded by CDK4 is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16 (INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16 (INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported.
Gene ID 1019
Alternative CDK4; Cyclin-dependent kinase 4; Cell division protein kinase 4; PSK-J3
Others Cdk4 Polyclonal Antibody detects endogenous levels of Cdk4 protein.
Accession P11802
Observed Band(KD) 35

Image & description

Fig.1. Western Blot analysis of MCF7 (1), K562 (2).

Fig.1. Western Blot analysis of MCF7 (1), K562 (2).

Fig.2. Immunofluorescence analysis of human stomach tissue. 1, Cdk4 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

Fig.2. Immunofluorescence analysis of human stomach tissue. 1, Cdk4 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

Fig.3. Immunofluorescence analysis of rat lung tissue. 1, Cdk4 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

Fig.3. Immunofluorescence analysis of rat lung tissue. 1, Cdk4 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

Fig.4. Immunohistochemical analysis of paraffin-embedded human brain, antibody was diluted at 1:100.

Fig.4. Immunohistochemical analysis of paraffin-embedded human brain, antibody was diluted at 1:100.

Fig.5. Immunohistochemical analysis of paraffin-embedded rat muscle, antibody was diluted at 1:100.

Fig.5. Immunohistochemical analysis of paraffin-embedded rat muscle, antibody was diluted at 1:100.

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